RT info:eu-repo/semantics/article
T1 Immunoproteomic analysis of the protective response obtained with subunit and commercial vaccines against Glässer’s disease in pigs
A1 Martínez Martínez, Sonia
A1 Frandoloso, Rafael
A1 Rodríguez Ferri, Elías Fernando
A1 Gil García, María Concepción
A1 Hernández Haro, Carolina
A1 Yubero-Delgado, Sheila
A1 Gutiérrez Martín, César Bernardo
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Glässer’s  disease
K1 Haemophilus  parasuis
K1 Antibody  response
K1 Pig
K1 Immunoproteomics
AB An immunoproteomic analysis of the protective response of subunit and commercial vaccinesin colostrumdeprived pigs against Glässer’s disease was carried out. A mixture ofproteins with affinity to porcine transferrin (PAPT) from Haemophilus parasuis Nagasakistrain (serovar 5) was inoculated intramuscularly (PAPTM) and intratracheally (PAPTCp),along with a commercial bacterin. PAPT were separated using 2 dimensional electrophoresis(2DE) gels and with them, 2DE Western blots were carried out. A total of 17 spots wereidentified as positive with sera of pigs from any of the three vaccinated groups, the highestnumber of immunoreactive proteins being detected in those having received PAPTCp.Among them, six proteins (FKBP-type peptidyl-prolyl cis-trans isomerase, neuraminidaseexo- -sialidase, xanthine-guanine phosphoribosyl transferase, CMP-N-acetylneuraminicacid synthetase, phenylalanyl-tRNA synthetase and glyceraldehyde 3-phosphate dehydrogenase)were found to be novel immunogens in H. parasuis. These proteins showed a highpotential as candidates in future subunit vaccines against Glässer’s disease. The three experimentalgroups developed specific systemic total IgG (IgGt), IgG1, IgG2 and IgM antibodiesafter immunizations. In addition, those receiving PAPTCp yielded a serum IgA response.
PB Elsevier
YR 2017
FD 2017-10-19
LK http://hdl.handle.net/10612/6870
UL http://hdl.handle.net/10612/6870
NO Veterinary Immunology and Immunopathology, vol. XXX
NO 14 p.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 24-abr-2024