RT info:eu-repo/semantics/preprint
T1 Immunomodulation of mesenchymal stem cells in discogenic pain
A1 Miguélez-Rivera, Laura
A1 Pérez Castrillo, Saúl
A1 González-Fernández, María Luisa
A1 Prieto Fernández, Julio Gabriel
A1 López-González, María Elisa
A1 García-Cosamalón, José
A1 Villar Suárez, María Vega
A2 Otros
K1 Bioquímica
K1 Conditioned medium
K1 Cytokines
K1 Discogenic pain
K1 Immunomodulation
K1 Mesenchymal stem cells
K1 Rat model
AB BACKGROUND CONTEXT: Back pain is a highly prevalent health problem in the world todayand has a great economic impact on health-care budgets. Intervertebral disc (IVD) degeneration hasbeen identified as a main cause of back pain. Inflammatory cytokines produced by macrophages ordisc cells in an inflammatory environment play an important role in painful progressive degenerationof IVD. Mesenchymal stem cells (MSCs) have shown to have immunosuppressive and antiinflammatoryproperties. Mesenchymal stem cells express a variety of chemokines and cytokinesreceptors having tropism to inflammation sites.PURPOSE: This study aimed to develop an in vitro controlled and standardized model of inflammationand degeneration of IVD with rat cells and to evaluate the protective and immunomodulatoryeffect of conditioned medium (CM) from the culture of MSCs to improve the conditions presentedin herniated disc and discogenic pain processes.STUDY DESIGN: This is an experimental study.METHODS: In this study, an in vitro model of inflammation and degeneration of IVD has beendeveloped, as well as the effectiveness of CM from the culture of MSCs.RESULTS: Conditioned medium from MSCs downregulated the expression of various proinflammatorycytokines produced in the pathogenesis of discogenic pain such as interleukin (IL)-1β, IL-6, IL-17,and tumor necrosis factor (TNF).CONCLUSION: Mesenchymal stem cells represent a promising alternative strategy in the treatmentof IVD degeneration inasmuch as there is currently no treatment which leads to a completeremission of long-term pain in the absence of drugs
PB Elsevier
YR 2017
FD 2017-12-22
LK http://hdl.handle.net/10612/7062
UL http://hdl.handle.net/10612/7062
NO The Spine Journal, 2017
NO 13 p.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 26-abr-2024