RT info:eu-repo/semantics/article
T1 Quinclorac-habituation of bean (Phaseolus vulgaris) cultured cells is related to an increase in their antioxidant capacity
A1 Largo-Gosens, Asier
A1 Castro Rodríguez, María de
A1 Alonso Simón, Ana
A1 García Angulo, Penélope
A1 Acebes Arranz, José Luis
A1 Encina García, Antonio Esteban
A1 Álvarez, Jesús Miguel
A2 Fisiologia Vegetal
K1 Fisiología
K1 Antioxidant activities
K1 Quinclorac
K1 Cell cultures
K1 Herbicide
K1 Oxidative damage
K1 Phaseolus vulgaris
AB The habituation of bean cells to quinclorac did not rely on cell wall modifications, contrary to what it was previously observed for the well-known cellulose biosynthesis inhibitors dichlobenil or isoxaben. The aim of the present study was to investigate whether or not the bean cells habituation to quinclorac is related to an enhancement of antioxidant activities involved in the scavenging capacity of reactive oxygen species. Treating non-habituated bean calluses with 10 μM quinclorac reduced the relative growth rate and induced a two-fold increase in lipid peroxidation. However, the exposition of quinclorac-habituated cells to a concentration of quinclorac up to 30 μM neither affected their growth rate nor increased their lipid peroxidation levels. Quinclorac-habituated calluses had significantly higher constitutive levels of three antioxidant activities (class-III peroxidase, glutathione reductase, and superoxide dismutase) than those observed in non-habituated calluses, and the treatment of habituated calluses with 30 μM quinclorac significantly increased the level of class III-peroxidase and superoxide dismutase. The results reported here indicate that the process of habituation to quinclorac in bean callus-cultured cells is related, at least partially, to the development of a stable antioxidant capacity that enables them to cope with the oxidative stress caused by quinclorac. Class-III peroxidase and superoxide dismutase activities could play a major role in the quinclorac-habituation. Changes in the antioxidant status of bean cells were stable, since the increase in the antioxidant activities were maintained in quinclorac-dehabituated cells
PB Elsevier
YR 2018
FD 2018-01-10
LK http://hdl.handle.net/10612/7073
UL http://hdl.handle.net/10612/7073
NO Plant Physiology and Biochemistry, 2016
NO 7 p.
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 26-abr-2024