RT info:eu-repo/semantics/article T1 Ectopic lignification in primary cellulose-deficient cell walls of maize cell suspension cultures A1 Mélida Martínez, Hugo A1 Largo-Gosens, Asier A1 Novo-Uzal, Esther A1 Santiago, Rogelio A1 Pomar, Federico A1 García García, Pedro AD 1957- A1 García Angulo, Penélope A1 Acebes Arranz, José Luis A1 Álvarez Fernández, Jesús Miguel A1 Encina García, Antonio Esteban A2 Fisiologia Vegetal K1 Fisiología K1 Cellulose K1 Dichlobenil K1 Ectopic lignin K1 Maize AB Maize (Zea mays L.) suspension-cultured cells withup to 70% less celluloseQ1 were obtained by stepwisehabituation to dichlobenil (DCB), a cellulose biosynthesisinhibitor. Cellulose deficiency was accompanied by markedchanges in cell wall matrix polysaccharides and phenolics asrevealed by Fourier transform infrared (FTIR) spectroscopy.Cell wall compositional analysis indicated that the cellulosedeficientcell walls showed an enhancement of highlybranched and cross-linked arabinoxylans, as well as anincreased content in ferulic acid, diferulates and pcoumaricacid, and the presence of a polymer that stainedpositive for phloroglucinol. In accordance with this, cellulosedeficientcell walls showed a fivefold increase in Klasontypelignin. Thioacidolysis/GC-MS analysis of cellulosedeficientcell walls indicated the presence of a ligninlikepolymer with a Syringyl/Guaiacyl ratio of 1.45, whichdiffered from the sensu stricto stress-related lignin that arosein response to short-term DCB-treatments. Gene expressionanalysis of these cells indicated an overexpression of genesspecific for the biosynthesis of monolignol units of lignin. Astudy of stress signaling pathways revealed an overexpressionof some of the jasmonate signaling pathway genes, whichmight trigger ectopic lignification in response to cell wallintegrity disruptions. In summary, the structural plasticity ofprimary cell walls is proven, since a lignification process ispossible in response to cellulose impoverishment PB Wiley YR 2018 FD 2018-01-11 LK http://hdl.handle.net/10612/7084 UL http://hdl.handle.net/10612/7084 NO Journal of Integrative Plant Biology, 2015 NO 18 p. DS BULERIA. Repositorio Institucional de la Universidad de León RD 28-mar-2024