RT info:eu-repo/semantics/article T1 Early habituation of maize (Zea mays) suspension-cultured cells to 2,6- diclorobenzonitrile is associated with the enhancement of antioxidant status A1 Largo-Gosens, Asier A1 Encina García, Antonio Esteban A1 Castro Rodríguez, María de A1 Mélida Martínez, Hugo A1 Acebes Arranz, José Luis A1 García Angulo, Penélope A1 Álvarez, Jesús Miguel A2 Fisiologia K1 Fisiología K1 Maize K1 Dichlobenil K1 Cellulose inhibitors K1 Oxidative damage K1 Antioxidant activities AB The cellulose biosynthesis inhibitor 2,6-diclorobenzonitrile (DCB) has been widely used to gain insights into cell wall composition and architecture. Studies of changes occurring during incipient habituation to DCB can provide information on early cell mechanisms leading to cope with DCB-induced stress. In this context, maize cultured cells with a reduced amount of cellulose were obtained by stepwise habituation to low DCB concentrations. The results reported here attempt to elucidate the putative role of an antioxidant strategy during incipient habituation. The short-term exposure to DCB of non-habituated maize cultured cells induced a substantial increase in oxidative damage. Concomitantly, short-term treated cells presented an increase in class III peroxidase and glutathione S-transferase activities and total glutathione content. Maize cells habituated to 0.3 – 1 μM DCB (incipient habituation) were characterised by a reduction in the relative cell growth rate, an enhancement of ascorbate peroxidase and class III peroxidase activities, and a net increment in total glutathione content. Moreover, these cell lines showed increased levels of glutathione S-transferase activity. Changes in antioxidant/detoxifying status enabled 0.3 and 0.5 μM DCB-habituated cells to control lipid peroxidation levels, but this was not the case of maize cells habituated to 1 μM DCB, which despite showing an increased antioxidant capacity were not capable of reducing the oxidative damage to control levels. The results reported here confirm that exposure and incipient habituation of maize cells to DCB are associated with an enhancement in antioxidant/detoxifying activities which could play a role in incipient DCB habituation of maize cultured cells PB Wiley YR 2018 FD 2018-01-11 LK http://hdl.handle.net/10612/7085 UL http://hdl.handle.net/10612/7085 NO Physiologia Plantarum, 2015 NO 30 p. DS BULERIA. Repositorio Institucional de la Universidad de León RD 29-mar-2024