RT info:eu-repo/semantics/article
T1 Genetic characterization of atypical enteropathogenic Escherichia coli (EPEC) strains isolated from ewes’ milk, sheep farms environment and humans by multilocus sequence typing and pulsed-field gel electrophoresis
A1 Otero, Verónica
A1 Rodríguez Calleja, José María
A1 Otero Carballeira, Andrés
A1 García López, María Luisa
A1 Santos Buelga, Jesús Ángel
A2 Tecnologia de los Alimentos
K1 Sanidad animal
K1 Veterinaria
K1 Multilocus sequence typing (MLST)
K1 Escherichia coli (EPEC)
K1 Sheep farms
K1 Ewes`milk
AB A collection of 81 isolates of enteropathogenic Escherichia coli (EPEC) was obtained from samples of bulk tank sheep milk(62 isolates), ovine feces (4 isolates), sheep farm environment (water, 4 isolates; air, 1 isolate), and human stool samples (9isolates). The strains were considered atypical EPEC organisms, carrying the eae gene without harboring the pEAF plasmid.Multilocus sequence typing (MLST) was carried out with seven housekeeping genes and 19 sequence types (ST) weredetected, with none of them having been previously reported for atypical EPEC. The most frequent ST included 41 strainsisolated from milk and human stool samples. Genetic typing by pulsed-field gel electrophoresis (PFGE) resulted in 57 patternswhich grouped in 24 clusters. Comparison of strains isolated from the different samples showed phylogenetic relationshipsbetween milk and human isolates and also between milk and water isolates. The results obtained show a possiblerisk for humans due to the presence of atypical EPEC in ewes’ milk and suggest a transmission route for this emergingpathogen through contaminated water.
PB American society for microbiology
YR 2018
FD 2018-02-03
LK http://hdl.handle.net/10612/7217
UL http://hdl.handle.net/10612/7217
NO Applied and environmental microbiology, vol. 79, n. 19
NO P. 5864-5869
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 24-abr-2024