RT info:eu-repo/semantics/article
T1 Detection of rainbow trout antibodies against viral haemorrhagic septicaemia virus (VHSV) by neutralisation test is highly dependent on the virus isolate used
A1 Fregeneda Grandes, Juan Miguel
A1 Olesen, Niels Jørgen
A2 Sanidad Animal
K1 Inmunología
K1 Sanidad animal
K1 Veterinaria
K1 Zoología
K1 Truchas
K1 Serología
K1 Inmunoglobulinas
AB Three serological tests, enzyme linked immunosorbent assay (ELISA), 50% plaque neutralisationtest (50%PNT) and Western blotting (WB), were used to detect antibodies against viralhaemorrhagic septicaemia virus (VHSV) in 50 rainbow trout broodstock from a rainbow trout farmendemically infected with VHS but with no clinical signs of infection. When the sera were examinedby 50%PNT using the VHSV reference isolate DK-F1 or the heat attenuated DK-F25 mutant strain,no neutralizing antibodies were found. In contrast, when one of the virus isolates from the farm(homologous virus) was used in the 50%PNT, 90% of the fish were found to be positive. By examininga panel of different VHSV isolates in 50%PNT, it was demonstrated that the virus isolate used astest antigen could significantly affect the sensitivity and titre determination in 50%PNT for detectionof rainbow trout antibodies against VHSV. When the sera were examined for the presence of VHSVantibodies by ELISA or WB, 61% were found to be positive. When conducting WB analysis, the viralglycoprotein was the protein most frequently recognized, followed by the viral nucleoprotein.
PB Inter-Research
YR 2018
FD 2018-11-09
LK http://hdl.handle.net/10612/9024
UL http://hdl.handle.net/10612/9024
NO Diseases of aquatic organisms, 2007, vol. 74, n. 2
NO P. 151-158
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 25-abr-2024