RT info:eu-repo/semantics/article
T1 Bacterial skin flora variation and in vitro inhibitory activity against Saprolegnia parasitica in brown and rainbow trout
A1 Fregeneda Grandes, Juan Miguel
A1 Carbajal González, María Teresa
A1 Suárez Ramos, Pilar Susana
A1 Rodríguez Cadenas, Fausto
A1 Aller Gancedo, José Miguel
A2 Sanidad Animal
K1 Inmunología
K1 Sanidad animal
K1 Zoología
K1 Saprolegnia parasítica
K1 Truchas
K1 Bacterias
AB Variations in the number and diversity of bacteria from the skin of brown trout Salmotrutta L. and rainbow trout Oncorhynchus mykiss Walbaum were surveyed from different riversand fish farms in northern Spain. In addition to determining bacterial populations in skin samplesof healthy fish, bacterial populations were determined from skin lesions (of brown trout only)infected with Saprolegnia parasitica, the causal agent of saprolegniosis. Mean bacterial countsfrom skin lesions of brown trout suffering from saprolegniosis were nearly 1000 times greater thanfrom the skin of uninfected brown and rainbow trout. More than 20 different genera of bacteriawere identified, with isolates of Aeromonas and Iodobacter being the predominant genera associatedwith saprolegniosis lesions. The in vitro inhibitory activity of 72 of these skin isolates wastested against S. parasitica using 3 different assays. These included (1) assessing the inhibition bybacteria of colony growth on agar media, (2) the inhibition of colony growth from colonized hempseeds in liquid media and (3) the inhibition of cyst germination in liquid media. Finally, the fungicidaleffect of the 24 most inhibitory bacterial species, and the inhibitory activity of their culturesupernatants, was tested in the same way. Isolates identified as Aeromonas piscicola, A. sobria,Pantoea agglomerans and Pseudomonas fluorescens achieved the highest inhibition against S.parasitica. Many of these inhibitory isolates were obtained primarily from skin lesions of fish withsaprolegniosis. It is suggested that some of these isolates might be useful in the biological controlof saprolegniosis.
PB Inter-Research
YR 2018
FD 2018-12-24
LK http://hdl.handle.net/10612/9203
UL http://hdl.handle.net/10612/9203
NO Diseases of aquatic organisms, 2011, vol. 96, n. 2
NO P. 126-135
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 19-abr-2024