RT info:eu-repo/semantics/article
T1 Adhesion to brown trout skin mucus, antagonism against cyst adhesion and pathogenicity to rainbow trout of some inhibitory bacteria against Saprolegnia parasitica
A1 Carbajal González, María Teresa
A1 Fregeneda Grandes, Juan Miguel
A1 González Palacios, Concepción
A1 Aller Gancedo, José Miguel
A2 Sanidad Animal
K1 Sanidad animal
K1 Veterinaria
K1 Zoología
K1 Truchas
K1 Saprolegnia parasítica
K1 Flora cutánea
AB Biological control of saprolegniosis with bacteria might be an alternative to the useof chemical compounds. Among criteria for the selection of such bacteria are their absence ofpathogenicity to fish and their ability to prevent adhesion of the pathogen to the skin mucus. Thepathogenicity to rainbow trout of 21 bacterial isolates with in vitro inhibitory activity againstSaprolegnia parasitica was studied. Fifteen of the isolates, identified as Aeromonas sobria, Pantoeaagglomerans, Pseudomonas fluorescens, Serratia fonticola, Xanthomonas retroflexus andYersinia kristensenii, were non-pathogenic when injected into rainbow trout. Their capacity toadhere to the skin mucus of male and female brown trout and to reduce the adhesion of S. parasiticacysts under exclusion, competition and displacement conditions was tested. The 15 bacterialisolates showed a low adhesion rate, ranging between 1.7% (for an A. sobria isolate) and 15.3%(a P. fluorescens isolate). This adhesion was greater in the case of mucus from male brown troutthan from females. Similarities in the adhesion to male mucus and other substrates and correlationto that observed to polystyrene suggest that adhesion to skin mucus does not depend on the substrate.A high percentage (88.9%) of the S. parasitica cysts adhered to the skin mucus of malebrown trout. Almost all of the bacteria reduced this adhesion ratio significantly under exclusionand competition conditions. However, only half of the isolates displaced cysts from skin mucus,and more bacterial cells were necessary for this effect. A novel method to study the adhesion of S.parasitica cysts to skin mucus of trout and their interactions with inhibitory bacteria is described.
PB Inter-Research
YR 2018
FD 2018-12-27
LK http://hdl.handle.net/10612/9280
UL http://hdl.handle.net/10612/9280
NO Diseases of aquatic organisms, 2013, vol. 104, n. 1
NO P. 35-44
DS BULERIA. Repositorio Institucional de la Universidad de León
RD 25-abr-2024