RT info:eu-repo/semantics/article T1 Bacterial community composition and diversity uncovered in experimental sludge treatment reed bed systems with different swine slurry hydraulic loadings A1 Arroyo Hernández, Paula A1 Sáenz de Miera Carnicer, Luis Enrique A1 Falagán Fernández, Jorge A1 Ansola González, Gemma A2 Ecologia K1 Ecología. Medio ambiente K1 High-throughput sequencing K1 Bacterial communities K1 STRB K1 Swine slurry K1 Nitrogen cycle AB Nowadays swine slurry management is a very important environmental issue. Sludge treatment reed bed systems are sludge treatment systems based on constructed wetlands.The present study characterizes via 16S rRNA gene high-throughput the bacterial communities contained in untreated swine slurry and treated swine slurry by means of mesocosm-scale sludge treatment reed beds receiving different slurry loadings.The bacterial community composition varied between the treated and untreated slurry, with a notable slurry loading influence also observed. Richness, diversity and ordination measurements of the studied communities evidenced profound differences between the untreated and treated swine slurry bacterial communities; and only slight differences among the treated swine slurry communities. Interestingly, the slurry loading allowed to define two groups: mesocosm communities residing in the highest hydraulic slurry loadings and other mesocosm communities. The pH value emerged as an important community composition, diversity and ordination predictor.The functional bacterial community composition was predicted through the in silico approach. Results confirmed that the main nitrogen cycle metabolic pathways were present in the mesocosm communities with ammonification and assimilatory nitrate reduction as the most commonly detected nitrogen pathways in treated swine slurry. PB Elsevier YR 2019 FD 2019-02-05 LK http://hdl.handle.net/10612/9502 UL http://hdl.handle.net/10612/9502 NO Ecological Engineering, vol. 123 NO P. 175-184 DS BULERIA. Repositorio Institucional de la Universidad de León RD 18-abr-2024