Mostra i principali dati dell'item
dc.contributor | Facultad de Ciencias Biologicas y Ambientales | es_ES |
dc.contributor.author | Martínez Pastor, Felipe | |
dc.contributor.author | Martínez, Félix | |
dc.contributor.author | Álvarez García, Mercedes | |
dc.contributor.author | Maroto Morales, Alejandro | |
dc.contributor.author | García Álvarez, Olga | |
dc.contributor.author | Soler, Ana J. | |
dc.contributor.author | Garde López-Brea, Julián | |
dc.contributor.author | Paz Cabello, Paulino de | |
dc.contributor.author | Anel Rodríguez, Luis | |
dc.contributor.other | Biologia Celular | es_ES |
dc.date | 2009-03-01 | |
dc.date.accessioned | 2019-04-16T22:42:11Z | |
dc.date.available | 2019-04-16T22:42:11Z | |
dc.date.issued | 2019-04-17 | |
dc.identifier.citation | Theriogenology, 2009, vol. 71, n. 4 | es_ES |
dc.identifier.other | https://www.sciencedirect.com/science/article/pii/S0093691X08006857#! | es_ES |
dc.identifier.uri | http://hdl.handle.net/10612/10288 | |
dc.description | P. 628-638 | es_ES |
dc.description.abstract | We tested extenders and freezing protocols for Iberian red deer semen. Samples were obtained by electroejaculation (10 stags), and analyzed for motility (CASA), viability (propidium ioide), acrosomal (PNA-FITC) and mitochondrial status (JC-1). Samples were diluted in extender, cooled and adjusted for glycerol (extender with higher glycerol concentration), brought to mL−1 and frozen. Four experiments were carried out, repeating sperm analysis after thawing to compare treatments. In a first experiment, seven samples were frozen using Triladyl® (20% egg yolk) and UL extender (Tes–Tris–fructose, 15% egg yolk, 4% glycerol). Triladyl® yielded higher motility after thawing. In a second trial, 17 samples were frozen using Triladyl®, Andromed®, Bioxcell®, and UL with 8% LDL (low-density lipoproteins). Triladyl® and Andromed® performed better than Bioxcell® on motility, and than UL-LDL on viability and acrosomal status. In a third experiment, the performance of freezing the sperm-rich ejaculate fraction versus the whole ejaculate was tested on nine samples. The sperm-rich ejaculate fraction not only rendered more motile and viable spermatozoa but also showed higher freezability (higher motile spermatozoa recovery). In a fourth experiment, we tried three modifications of the freezing protocol, for improving the freezability of low concentration samples: prior removal of seminal plasma; replacing extender (second fraction) for pure glycerol to reduce dilution; and performing only the dilution, not the second dilution. No differences were found, although only three samples could be used. Both Triladyl® and Andromed® were deemed appropriate for freezing Iberian red deer semen, and the rich fraction should be selected for freezing. | es_ES |
dc.language | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.subject | Veterinaria | es_ES |
dc.subject.other | Sperm cryopreservation | es_ES |
dc.subject.other | Electroejaculation | es_ES |
dc.subject.other | Extenders | es_ES |
dc.subject.other | Red deer | es_ES |
dc.subject.other | Semen | es_ES |
dc.title | Cryopreservation of Iberian red deer (Cervus elaphus hispanicus) spermatozoa obtained by electroejaculation | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.peerreviewed | SI | es_ES |
Files in questo item
Questo item appare nelle seguenti collezioni
-
Untitled [5590]