dc.contributor | Facultad de Ciencias Biologicas y Ambientales | es_ES |
dc.contributor.author | Cardoza, Rosa E. | |
dc.contributor.author | McCormick, Susan P. | |
dc.contributor.author | Izquierdo-Bueno, Inmaculada | |
dc.contributor.author | Martínez-Reyes, Natalia | |
dc.contributor.author | Lindo, Laura | |
dc.contributor.author | Brown, Daren W. | |
dc.contributor.author | Collado, Isidro G. | |
dc.contributor.author | Proctor, Robert H. | |
dc.contributor.author | Gutiérrez Martín, Santiago | |
dc.contributor.other | Microbiologia | es_ES |
dc.date | 2022 | |
dc.date.accessioned | 2022-09-28T10:56:05Z | |
dc.date.available | 2022-09-28T10:56:05Z | |
dc.identifier.citation | Cardoza, R.E., McCormick, S.P., Izquierdo-Bueno, I. et al. Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum. Appl Microbiol Biotechnol (2022). https://doi.org/10.1007/s00253-022-12182-9 | es_ES |
dc.identifier.issn | 0175-7598 | |
dc.identifier.uri | http://hdl.handle.net/10612/15149 | |
dc.description | https://link.springer.com/article/10.1007/s00253-022-12182-9 | es_ES |
dc.description.abstract | [EN] The fungus Trichoderma arundinaceum exhibits biological control activity against crop diseases caused by other fungi. Two mechanisms that likely contribute to this activity are upregulation of plant defenses and production of two types of antifungal secondary metabolites: the sesquiterpenoid harzianum A (HA) and the polyketide-derived aspinolides. The goal of the current study was to identify aspinolide biosynthetic genes as part of an effort to understand how these metabolites contribute to the biological control activity of T. arundinaceum. Comparative genomics identified two polyketide synthase genes (asp1 and asp2) that occur in T. arundinaceum and Aspergillus ochraceus, which also produces aspinolides. Gene deletion and biochemical analyses in T. arundinaceum indicated that both genes are required for aspinolide production: asp2 for formation of a 10-member lactone ring and asp1 for formation of a butenoyl subsituent at position 8 of the lactone ring. Gene expression and comparative genomics analyses indicated that asp1 and asp2 are located within a gene cluster that occurs in both T. arundinaceum and A. ochraceus. A survey of genome sequences representing 35 phylogenetically diverse Trichoderma species revealed that intact homologs of the cluster occurred in only two other species, which also produced aspinolides. An asp2 mutant inhibited fungal growth more than the wild type, but an asp1 mutant did not, and the greater inhibition by the asp2 mutant coincided with increased HA production. These findings indicate that asp1 and asp2 are aspinolide biosynthetic genes and that loss of either aspinolide or HA production in T. arundinaceum can be accompanied by increased production of the other metabolite(s). | es_ES |
dc.language | eng | es_ES |
dc.publisher | Springer | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Biología | es_ES |
dc.subject.other | Aspinolides | es_ES |
dc.subject.other | Trichoderma | es_ES |
dc.subject.other | Trichothecenes | es_ES |
dc.subject.other | Metabolomics | es_ES |
dc.subject.other | Gene clusters | es_ES |
dc.subject.other | Comparative genomics | es_ES |
dc.title | Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1007/s00253-022-12182-9 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info: eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-099600-B-I00/AISLAMIENTO DE CEPAS DE TRICHODERMA PRODUCTORAS DE TRICHOTECENOS A PARTIR DE CULTIVOS DE JUDIA Y ESTUDIO DE SU EFECTO EN LA DEFENSA DE LA PLANTA FRENTE A ENFERMEDADES FUNGICAS | |
dc.relation.projectID | info: eu-repo/grantAgreement/MCIN/ AEI/10.13039/501100011033 | |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1432-0614 | |
dc.journal.title | Applied Microbiology and Biotechnology | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 2415 Biología Molecular | es_ES |
dc.subject.unesco | 2414 Microbiología | es_ES |
dc.description.project | Publicación en abierto financiada por el Consorcio de Bibliotecas Universitarias de Castilla y León (BUCLE), con cargo al Programa Operativo 2014ES16RFOP009 FEDER 2014-2020 DE CASTILLA Y LEÓN, Actuación:20007-CL - Apoyo Consorcio BUCLE | es_ES |