dc.contributor | Facultad de Ciencias Biologicas y Ambientales | es_ES |
dc.contributor.author | Fuente, Álvaro de la | |
dc.contributor.author | Martín Martín, Juan Francisco | |
dc.contributor.author | Rodríguez García, Antonio | |
dc.contributor.author | Liras Padín, Paloma | |
dc.contributor.other | Microbiologia | es_ES |
dc.date | 2004-10-01 | |
dc.date.accessioned | 2024-01-23T08:49:44Z | |
dc.date.available | 2024-01-23T08:49:44Z | |
dc.identifier.citation | Fuente, A. de la, Martín, J. F., Rodríguez-García, A. & Liras, P. (2004). Two proteins with ornithine acetyltransferase activity show different functions in Streptomyces clavuligerus: Oat2 modulates clavulanic acid biosynthesis in response to arginine. Journal of Bacteriology, 186(19), 6501-6507. https://doi.org/10.1128/JB.186.19.6501-6507.2004 | es_ES |
dc.identifier.issn | 0021-9193 | |
dc.identifier.other | https://journals.asm.org/doi/10.1128/jb.186.19.6501-6507.2004 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/17721 | |
dc.description.abstract | [EN] The oat2 gene, located in the clavulanic acid gene cluster in Streptomyces clavuligerus, is similar to argJ, which encodes N-acetylornithine:glutamic acid acetyltransferase activity. Purified proteins obtained by expression in Escherichia coli of the argJ and oat2 genes of S. clavuligerus posses
N-acetyltransferase activity. The kinetics and substrate specificities of both proteins are very similar. Deletion of the oat2 gene did not affect the total N-acetylornithine transferase activity and slightly reduced the formation of clavulanic acid under standard culture conditions. However, the oat2 mutant produced more clavulanic acid than the parental strain in cultures supplemented with high levels (above 1 mM) of arginine. The purified S. clavuligerus ArgR protein bound the arginine box in the oat2 promoter, and the expression of oat2 was higher in mutants with a disruption in argR (arginine-deregulated), confirming that the Arg boxes of oat2 are functional in vivo. Our results suggest that the Oat2 protein or one of its reaction products has a regulatory role that modulates clavulanic acid biosynthesis in response to high arginine concentrations | es_ES |
dc.language | eng | es_ES |
dc.publisher | American Society for Microbiology | es_ES |
dc.subject | Biotecnología | es_ES |
dc.subject.other | Ornithine N-acetyltransferase | es_ES |
dc.subject.other | Streptomyces clavuligerus | es_ES |
dc.subject.other | Clavulanic acid biosynthesis | es_ES |
dc.subject.other | Arginine regulation | es_ES |
dc.subject.other | ArgR | es_ES |
dc.subject.other | Oat2 | es_ES |
dc.title | Two proteins with ornithine acetyltransferase activity show different functions in Streptomyces clavuligerus: Oat2 modulates clavulanic acid biosynthesis in response to arginine | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1128/JB.186.19.6501-6507.2004 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MICYT//BIO2000-272/ES | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/embargoedAccess | es_ES |
dc.identifier.essn | 1098-5530 | |
dc.journal.title | Journal of Bacteriology | es_ES |
dc.volume.number | 186 | es_ES |
dc.issue.number | 19 | es_ES |
dc.page.initial | 6501 | es_ES |
dc.page.final | 6507 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 2414.03 Metabolismo Bacteriano | es_ES |
dc.subject.unesco | 2415.01 Biología Molecular de Microorganismos | es_ES |
dc.description.project | This work was supported by grant BIO2000-272 and a fellowship (to A. de la Fuente) from the Spanish Ministry of Science and Technology (Madrid, Spain). We thank Rosario Pérez-Redondo for her help with RNA experiments. | es_ES |