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Título
Modeling Dynamics of Human Gut Microbiota Derived from Gluten Metabolism: Obtention, Maintenance and Characterization of Complex Microbial Communities
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
International Journal of Molecular Sciences
Número de la revista
7
Datos de la obra
Carnicero-Mayo, Y., Sáenz de Miera, L. E., Ferrero, M. Á., Navasa, N., & Casqueiro, J. (2024). Modeling dynamics of human gut microbiota derived from gluten metabolism: obtention, maintenance and characterization of complex microbial communities. International Journal of Molecular Sciences, 25(7), Article e4013. https://doi.org/10.3390/ijms25074013
Editor
MDPI
Fecha
2024
ISSN
1661-6596
Abstract
[EN] Western diets are rich in gluten-containing products, which are frequently poorly digested. The human large intestine harbors microorganisms able to metabolize undigested gluten fragments that have escaped digestion by human enzymatic activities. The aim of this work was obtaining and culturing complex human gut microbial communities derived from gluten metabolism to model the dynamics of healthy human large intestine microbiota associated with different gluten forms. For this purpose, stool samples from six healthy volunteers were inoculated in media containing predigested gluten or predigested gluten plus non-digested gluten. Passages were carried out every 24 h for 15 days in the same medium and community composition along time was studied via V3–V4 16S rDNA sequencing. Diverse microbial communities were successfully obtained. Moreover, communities were shown to be maintained in culture with stable composition for 14 days. Under non-digested gluten presence, communities were enriched in members of Bacillota, such as Lachnospiraceae, Clostridiaceae, Streptococcaceae, Peptoniphilaceae, Selenomonadaceae or Erysipelotrichaceae, and members of Actinomycetota, such as Bifidobacteriaceae and Eggerthellaceae. Contrarily, communities exposed to digested gluten were enriched in Pseudomonadota. Hence, this study shows a method for culture and stable maintenance of gut communities derived from gluten metabolism. This method enables the analysis of microbial metabolism of gluten in the gut from a community perspective
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ID proyecto
- info:eu-repo/grantAgreement/AEI/ Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2020-119044GB-I00/ES/Caracterización de la firma microbiana asociada a la enfermedad celíaca. Impacto de la dieta en la inmunogenicidad del gluten
- info:eu-repo/grantAgreement/JCYL//LE015P20/ES/Estudio del impacto de la dieta en el metabolismo microbiano del gluten y la inmunogenicidad asociada a la enfermedad celíaca
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