RT info:eu-repo/semantics/article
T1 Improving the effect of incubation and oxidative stress on thawed spermatozoa from red deer by using different antioxidant treatments
A1 Domínguez Rebolledo, Álvaro Efrén
A1 Fernández Santos, María Rocío
A1 Bisbal Vigo, Alfonso
A1 Ros Santaella, José Luis
A1 Ramón Fernández, Manuel
A1 Carmona Delgado, Manuel
A1 Martínez Pastor, Felipe
A1 Garde López-Brea, Julián
A2 Biologia Celular
K1 Veterinaria
K1 Crocin
K1 Lipoic acid
K1 Melatonin
K1 Trolox
AB Antioxidants could improve sperm media, extending the viability of spermatozoa and protecting their DNA. The protective ability of lipoic acid, melatonin, Trolox and crocin was tested on red deer spermatozoa incubated at 37°C. Cryopreserved spermatozoa were thawed and incubated with 1 mM or 0.1 mM of each antioxidant, with or without oxidative stress (100 μM Fe2+). Motility (CASA), viability, mitochondrial membrane potential and acrosomal status were assessed. Lipoperoxidation (malondialdehyde production), intracellular reactive oxygen species (ROS) and DNA status (TUNEL) were checked at 4 h. Incubation alone increased ROS and decreased motility. Oxidative stress intensified these effects, increasing lipoperoxidation and DNA damage. Lipoic acid had little protective effect, whereas 1 mM melatonin showed limited protection. Trolox lowered ROS and lipoperoxidation both in oxidised and non-oxidised samples. In oxidised samples, Trolox prevented DNA and acrosomal damage, and ameliorated motility. Crocin at 1 mM showed similar results to Trolox, but noticeably stimulated motility and had no effect on lipoperoxidation. In a second experiment, a broader range of crocin and melatonin concentrations were tested, confirming the effects of crocin (positive effects noticeable at 0.5–0.75 mM), but showing an increase in lipoperoxidation at 2 mM. Melatonin was increasingly effective at 2.5 and 5 mM (ROS, lipoperoxidation and DNA status). Crocin seems a promising new antioxidant, but its particular effects on sperm physiology must be further studied, especially the consequences of motility stimulation and confirming its effect on lipoperoxidation. Melatonin might be useful at relatively high concentrations, compared to Trolox.
PB CSIRO
YR 2019
FD 2019-04-15
LK http://hdl.handle.net/10612/10222
UL http://hdl.handle.net/10612/10222
NO Reproduction, Fertility and Development, 2010, vol. 22, n. 5
NO P. 856-870
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 7, 2024