RT info:eu-repo/semantics/article
T1 Seminal plasma improves cryopreservation of Iberian red deer epididymal sperm
A1 Martínez Pastor, Felipe
A1 Anel Rodríguez, Luis
A1 Guerra, Camino
A1 Álvarez García, Mercedes
A1 Soler, Ana J.
A1 Garde López-Brea, Julián
A1 Chamorro Álvarez, César Ángel
A1 Paz Cabello, Paulino de
A2 Biologia Celular
K1 Veterinaria
K1 Red deer
K1 Epididymal sperm
K1 Seminal plasma
K1 Cryopreservation
K1 Wildlife preservation
AB We tested the protective action of seminal plasma on epididymal spermatozoa from Iberian red deer, especially considering cryopreservation, as a means for germplasm banking improvement. We obtained seminal plasma by centrifuging electroejaculated semen, and part of it was thermically inactivated (denatured plasma; 55 °C 30 min). Epididymal samples (always at 5 °C) were obtained from genitalia harvested after regulated hunting, and pooled for each assay (five in total). We tested three seminal plasma treatments (mixing seminal plasma with samples 2:1): no plasma, untreated plasma and denatured plasma; and four incubation treatments: 32 °C 15 min, 5 °C 15 min, 5 °C 2 h and 5 °C 6 h. After each incubation, samples were diluted 1:1 with extender: Tes-Tris-Fructose, 10% egg yolk, 4% glycerol; equilibrated for 2 h at 5 °C, extended down to 108 spz./mL and frozen. Sperm quality was evaluated before 1:1 dilution, before freezing and after thawing the samples, assessing motility (CASA) and viability (percentage of viable and acrosome-intact spermatozoa; PI/PNA-FITC and fluorescent microscopy). Plasma treatment, both untreated and denatured, rendered higher viability before freezing and higher results for most parameters after thawing. The improvement was irrespective of incubation treatment, except for viability, which rendered slightly different results for untreated and denatured plasma. This may be due to the presence of thermolabile components. We still have to determine the underlying mechanisms involved in this protection. These results might help to improve the design of cryopreservation extenders for red deer epididymal sperm.
PB Elsevier
YR 2019
FD 2019-04-23
LK http://hdl.handle.net/10612/10605
UL http://hdl.handle.net/10612/10605
NO Theriogenology, 2006, vol. 66, n. 8
NO P. 1847-1856
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 7, 2024