RT info:eu-repo/semantics/article
T1 Outlining adequate protocols for Lidia bull epididymal storage and sperm cryopreservation: use of glycerol, dimethylformamide and N-acetylcysteine
A1 Matilla Pinto, Elvira
A1 González Fernández, Lauro
A1 Martínez Pastor, Felipe
A1 Hernández Rollán, Nuria
A1 Tobajas Vega, Carolina
A1 Calle Guisado, Violeta
A1 Mijares Gordún, José
A1 Sánchez Margallo, Francisco Miguel
A1 Álvarez Miguel, Ignacio Santiago
A1 Macías García, Beatriz
A2 Biologia Celular
K1 Veterinaria
K1 Fighting bull
K1 Epididymal sperm
K1 Sperm freezing extenders
K1 Antioxidants
K1 Cooling
AB The Lidia bovine breed is an important hallmark of the Spanish cattle industry. Bulls are selected based upon aggressiveness and epididymal sperm cryopreservation is the way to obtain and store their genetics. There are not specifically designed protocols yet to perform Lidia bull sperm cryopreservation. The present study aimed to determine if a tris-fructose-citrate-egg yolk (20% v/v; TFY) extender supplemented with 7% glycerol (TFY1) or 3.5% glycerol plus 3.5% dimethylformamide (DMF; TFY2) are suitable media for cryopreservation of epididymal Lidia bull sperm. Moreover, the effect of N-acetylcysteine (NAC), a potent antioxidant, was evaluated. The epididymis were stored at 4°C for 24, 48, 72 or 96 h, and both freezing media were tested as such or supplemented with 1 or 2.5 mM of NAC. Our data demonstrated that post-thaw viability was well maintained (TFY1: 50.8% ± 1.9 at 24 h and 52.4% ± 0.8 at 96 h and TFY2: 52.6% ± 1.6 at 24 h and 56.1% ± 1.8 at 96 h; mean % ± SEM; p>0.05) as also were total and progressive sperm motility, high mitochondrial membrane potential, ROS production, DNA status and acrosomal intactness of Lidia bull sperm up to 96 h of epididymal storage, all extender variations being similar (p>0.05). In conclusion, the use of TFY medium supplemented either with 7% glycerol alone or the combination of 3.5% glycerol and 3.5% DMF were equally safe choices for epididymal Lidia bull sperm cryopreservation, and NAC addition did not significantly improve sperm post-thaw quality.
PB Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
YR 2019
FD 2019-05-13
LK http://hdl.handle.net/10612/10719
UL http://hdl.handle.net/10612/10719
NO Spanish Journal of Agricultural Research, 2017,  vol. 15, n. 3
NO P. 1-10
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 7, 2024