RT info:eu-repo/semantics/article
T1 Efficacy of heat-inactivated Mycobacterium bovis vaccine delivered to European badgers (Meles meles) through edible bait
A1 Juste, Ramón A.
A1 Blanco-Vázquez, Cristina
A1 Barral, Marta
A1 Prieto, José Miguel
A1 Varela-Castro, Lucía
A1 Lesellier, Sandrine
A1 Dave, Dipesh
A1 Sevilla, Iker A.
A1 Martín Ezquerra, Ana Belén
A1 Adriaensen, Hans
A1 Herrero-García, Gloria
A1 Garrido, Joseba M.
A1 Casais, Rosa
A1 Balseiro Morales, Ana María
A2 Sanidad Animal
K1 Sanidad animal
K1 Tuberculosis
K1 Badger
K1 Mycobacterium bovis heat-inactivated bait oral vaccine
K1 HIMB
K1 Efficacy
AB [EN] Badgers (Meles meles) are a major tuberculosis (TB) reservoir in Europe, with the potential to transmit infection to cattle. Here we assessed whether a recently described oral tuberculosis vaccine based on heat-inactivated Mycobacterium bovis (HIMB), delivered as edible baits, can protect badgers from infection. Eight badgers were given individually five baits, each one consisting of a ball of peanut butter, natural peanut and oat flakes including a dose of the vaccine containing 5 × 107 colony-forming units. In parallel, a control group of seven badgers did not receive the vaccine. One month and a half later a second dose of the vaccine was offered to the vaccinated group. Ninety-four days after the second dose, all badgers were challenged with M. bovis (103 colony-forming units per animal) delivered endobronchially to the right middle lung lobe. Clinical, immunological, pathological and bacteriological variables were measured throughout the whole study to assess the efficacy of the vaccine. Two vaccinated animals showed high bacterial load of M. bovis and worsening of pathological lesions of TB. Conversely, the other six vaccinated animals showed slight improvement in bacterial load and pathology with respect to the control group. These results suggest that delivering the TB vaccine via food bait can partially protect wild badger populations, although vaccination can lead to either protection or tolerization, likely depending on the animal's immune status and general condition at the time of vaccination. Further optimization of the vaccination trial/strategy is needed to reduce the rate of tolerization, such as altering vaccine dose, number of doses, type of bait, use of adjuvants or route of administration.
PB Elsevier
SN 2405-8440
LK https://hdl.handle.net/10612/17096
UL https://hdl.handle.net/10612/17096
NO Juste, R. A., Blanco-Vázquez, C., Barral, M., Prieto, J. M., Varela-Castro, L., Lesellier, S., Dave, D., Sevilla, I. A., Martín Ezquerra, A. B., Adriaensen, H., Herrero-García, G., Garrido, J. M., Casais, R., y  Balseiro, A. (2023). Efficacy of heat-inactivated Mycobacterium bovis vaccine delivered to European badgers (Meles meles) through edible bait. Heliyon, 9(9), e19349. https://doi.org/10.1016/j.heliyon.2023.e19349
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 6, 2024