RT info:eu-repo/semantics/article
T1 Analysis of the interaction between tryptophan-related compounds and ATP-binding cassette transporter G2 (ABCG2) using targeted metabolomics
A1 Garcia-Lino, Alba M.
A1 Gomez-Gomez, Alex
A1 Garcia-Mateos, Dafne
A1 de la Fuente, Alvaro
A1 Álvarez de Felipe, Ana Isabel
A1 Pozo, Oscar J.
A1 Merino Peláez, Gracia
A2 Fisiologia
K1 Fisiología
K1 ATP binding cassette transporter G2
K1 In vivo
K1 Tryptophan
K1 Metabolome
K1 Mice
K1 3109 Ciencias Veterinarias
AB ATP-binding cassette transporter G2 (ABCG2) is involved in the secretion of several compounds in milk. The in vitro and in vivo interactions between tryptophan-related compounds and ABCG2 were investigated. The tryptophan metabolome was determined by  liquid chromatography-tandem mass spectrometry in milk and plasma from wild-type and Abcg2-/- mice as well as dairy cows carrying the ABCG2 Y581S polymorphism (Y/S) and noncarrier animals (Y/Y). The milk-to-plasma ratios of tryptophan, kynurenic acid,  kynurenine, anthranilic acid, and xanthurenic acid were higher in wild-type mice than in Abcg2-/- mice. The ratio was 2-fold higher in Y/S than in Y/Y cows for kynurenine. In vitro transport assays confirmed that some of these compounds were in vitro substrates of the transporter and validated the differences observed between the two variants of the bovine protein. These findings show that the secretion of metabolites belonging to the kynurenine pathway into milk is mediated by ABCG2.
PB Elsevier
SN 0308-8146
LK https://hdl.handle.net/10612/17471
UL https://hdl.handle.net/10612/17471
NO Garcia-Lino, A. M., Gomez-Gomez, A., Garcia-Mateos, D., de la Fuente, A., Alvarez, A. I., Pozo, O. J., & Merino, G. (2021). Analysis of the interaction between tryptophan-related compounds and ATP-binding cassette transporter G2 (ABCG2) using targeted metabolomics. Food Chemistry, 344, Article e128665. https://doi.org/10.1016/J.FOODCHEM.2020.128665
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 6, 2024