RT info:eu-repo/semantics/article
T1 Towards the design of universal immunosurfaces for SPR-based assays: A review
A1 Lechuga, L.M.
A1 Mauriz García, Elba
A1 Gonzalez Fernandez, Maria Jesus
A2 Algebra
K1 Química
K1 SPR
K1 Biofunctionalizacion
K1 Antibody immobilization
K1 Special immunosurfaces
AB Surface biofunctionalization, including chemical activation and attachment of the bioreceptor, is an essential step to provide reliable detection of biomolecular binding events monitored by Surface Plasmon Resonance (SPR), the most employed optical biosensor, and other biosensor techniques. Recent progress in the area of immobilization procedures are aimed at producing reproducible interfacial surfaces that enable the sensitive and specific recognition of the analyte. Antibodies are still the most employed bioreceptors for SPR assays. A wide range of strategies have been proposed to maximize the SPR immunosensor performance by controlling the stability and orientation of the immobilized antibody. This article reviews the most recent advancements in random and oriented antibody immobilization approaches for SPR biosensing applications, with a special focus on the research that have been done to find universal linkers, which can allow the use of the same functionalized surface for different applications.
PB Elsevier
SN 0165-9936
LK https://hdl.handle.net/10612/17901
UL https://hdl.handle.net/10612/17901
NO MaurizE.,García-FernándezM.C.,LechugaL.M..Towardsthe designofuniversal immunosurfacesforSPR-basedassays:A review.TrAC-TrendsinAnalyticalChemistry,(2016).79.:191-.10.1016/j.trac.2016.02.006,
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 7, 2024