RT info:eu-repo/semantics/article
T1 Transcriptomic analysis of Streptomyces coelicolor differentiation in solid sporulating cultures: first compartmentalized and second multinucleated mycelia have different and distinctive transcriptomes
A1 Yagüe Menéndez, Paula
A1 Rodríguez García, Antonio
A1 López García, María Teresa
A1 Martín Martín, Juan Francisco
A1 Rioseras de Bustos, Beatriz
A1 Sánchez Martín, Jesús
A1 Manteca Fernández, Ángel
A2 Microbiologia
K1 Biología
K1 Streptomyces
K1 Streptomyces coelicolor
K1 Morphological differentiation
K1 Antibotics biosynthesis regulation
K1 Transcriptomics
K1 2415.01 Biología Molecular de Microorganismos
K1 2414.02 Fisiología Bacteriana
AB [EN] Streptomycetes are very important industrial bacteria, which produce two thirds of all clinically relevant secondary metabolites. They have a complex developmental-cycle in which an early compartmentalized mycelium (MI) differentiates to a multinucleated mycelium (MII) that grows inside the culture medium (substrate mycelium) until it starts to growth into the air (aerial mycelium) and ends up forming spores. Streptomyces developmental studies have focused mainly on the later stages of MII differentiation (aerial mycelium and sporulation), with regulation of pre-sporulation stages (MI/MII transition) essentially unknown. This work represents the first study of the Streptomyces MI transcriptome, analyzing how it differs from the MII transcriptome. We have used a very conservative experimental approach to fractionate MI from MII and quantify gene expressions. The expression of well characterized key developmental/metabolic genes involved in bioactive compound production (actinorhodin, undecylprodigiosin, calcium-dependent antibiotic, cpk, geosmin) or hydrophobic cover formation-sporulation (bld, whi, wbl, rdl, chp, ram) was correlated with MII differentiation. Additionally, 122 genes conserved in the Streptomyces genus, whose biological function had not been previously characterized, were found to be differentially expressed (more than 4-fold) in MI or MII. These genes encoded for putative regulatory proteins (transcriptional regulators, kinases), as well as hypothetical proteins. Knowledge about differences between the MI (vegetative) and MII (reproductive) transcriptomes represents a huge advance in Streptomyces biology that will make future experiments possible aimed at characterizing the biochemical pathways controlling pre-sporulation developmental stages and activation of secondary metabolism in Streptomyces
PB Public Library of Science
LK https://hdl.handle.net/10612/17982
UL https://hdl.handle.net/10612/17982
NO Yagüe, P., Rodríguez-García, A., López-García, M.T., Martín, J.F., Rioseras, B., Sánchez, J., & Manteca, A. (2013) Transcriptomic analysis of Streptomyces coelicolor differentiation in solid sporulating cultures: first compartmentalized and second multinucleated mycelia have different and distinctive transcriptomes. PLoS ONE 8(3), Article e60665. https://doi.org/10.1371/journal.pone.0060665
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 6, 2024