RT info:eu-repo/semantics/article
T1 The dynamic architecture of the metabolic switch in Streptomyces coelicolor
A1 Nieselt, Kay
A1 Battke, Florian
A1 Herbig, Alexander
A1 Bruheim, Per
A1 Wentzel, Alexander
A1 Jakobsen, Øyvind M
A1 Sletta, Håvard
A1 Alam, Mohammad T
A1 Merlo, Maria E
A1 Moore, Jonathan
A1 Omara, Walid AM
A1 Morrissey, Edward R
A1 Juarez-Hermosillo, Miguel A
A1 Rodríguez García, Antonio
A1 Nentwich, Merle
A1 Thomas, Louise
A1 Iqbal, Mudassar
A1 Legaie, Roxane
A1 Gaze, William H
A1 Challis, Gregory L
A1 Jansen, Ritsert C
A1 Dijkhuizen, Lubbert
A1 Rand, David A
A1 Wild, David L
A1 Bonin, Michael
A1 Reuther, Jens
A1 Wohlleben, Wolfgang
A1 Smith, Margaret CM
A1 Burroughs, Nigel J
A1 Martín Martín, Juan Francisco
A1 Hodgson, David A
A1 Takano, Eriko
A1 Breitling, Rainer
A1 Ellingsen, Trond E
A1 Wellington, Elizabeth MH
A2 Microbiologia
K1 Biología
K1 Streptomyces coelicolor
K1 Phosphate regulation
K1 Transcriptomics
K1 Antibotics biosynthesis regulation
K1 Metabolic switch
K1 2414.02 Fisiología Bacteriana
K1 2415.01 Biología Molecular de Microorganismos
AB [EN] Background: During the lifetime of a fermenter culture, the soil bacterium S. coelicolor undergoes a major metabolic switch from exponential growth to antibiotic production. We have studied gene expression patterns during this switch, using a specifically designed Affymetrix genechip and a high-resolution time-series of fermenter-grown samples.Results: Surprisingly, we find that the metabolic switch actually consists of multiple finely orchestrated switching events. Strongly coherent clusters of genes show drastic changes in gene expression already many hours before the classically defined transition phase where the switch from primary to secondary metabolism was expected. The main switch in gene expression takes only 2 hours, and changes in antibiotic biosynthesis genes are delayed relative to the metabolic rearrangements. Furthermore, global variation in morphogenesis genes indicates an involvement of cell differentiation pathways in the decision phase leading up to the commitment to antibiotic biosynthesis.Conclusions: Our study provides the first detailed insights into the complex sequence of early regulatory events during and preceding the major metabolic switch in S. coelicolor, which will form the starting point for future attempts at engineering antibiotic production in a biotechnological setting
PB BioMed Central
SN 1471-2164
LK https://hdl.handle.net/10612/18079
UL https://hdl.handle.net/10612/18079
NO Nieselt, K., Battke, F., Herbig, A., Bruheim, P., Wentzel, A., Jakobsen, Ø. M., Sletta, H., Alam, M. T., Merlo, M. E., Moore, J., Omara, W. A. M., Morrissey, E. R., Juarez-Hermosillo, M. A., Rodríguez-García, A., Nentwich, M., Thomas, L., Iqbal, M., Legaie, R., Gaze, W. H., et al. (2010). The dynamic architecture of the metabolic switch in Streptomyces coelicolor. BMC Genomics, 11, Article e10. https://doi.org/10.1186/1471-2164-11-10
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 7, 2024