RT info:eu-repo/semantics/article
T1 Bos taurus and Cervus elaphus as Non-Seasonal/Seasonal Models for the Role of Melatonin Receptors in the Spermatozoon
A1 Fernández Alegre, Estela
A1 Lacalle Fernández, Estíbaliz
A1 Soriano Úbeda, Cristina de las Mercedes
A1 González Montaña, José Ramiro
A1 Domínguez Fernández de Tejerina, Juan Carlos
A1 Casao, Adriana
A1 Martínez Pastor, Felipe
A2 Biologia Celular
K1 Biología
K1 Veterinaria
K1 Melatonin
K1 Membrane receptor
K1 GPCR
K1 Agonist
K1 Antagonist
K1 Spermatozoon
K1 Bull
K1 Deer
K1 Seasonality
K1 Reproduction
K1 3109 Ciencias Veterinarias
K1 2415 Biología Molecular
AB [EN] Melatonin is crucial in reproduction due its antioxidant, hormonal, and paracrine action. Melatonin membrane receptors (MT1/MT2) have been confirmed on spermatozoa from several species, but functionality studies are scarce. To clarify their role in ruminants as reproductive models, bull (Bos taurus, non-seasonal) and red deer (Cervus elaphus, highly seasonal) spermatozoa were analyzed after 4 h of incubation (38 °C, capacitating media) in 10 nM melatonin, MT1/MT2 agonists (phenylmelatonin and 8M-PDOT), and antagonists (luzindole and 4P-PDOT). Motility and functionality (flow cytometry: Viability, intracellular calcium, capacitation status, reactive oxygen species (ROS) production, and acrosomal and mitochondrial status) were assessed. In bull, MT1 was related to sperm viability preservation, whereas MT2 could modulate cell functionality to prevent excess ROS produced by the mitochondria; this action could have a role in modulating sperm capacitation. Deer spermatozoa showed resistance to melatonin and receptor activation, possibly because the samples were of epididymal origin and collected at the breeding season’s peak, with high circulating melatonin. However, receptors could be involved in mitochondrial protection. Therefore, melatonin receptors are functional in the spermatozoa from bull and deer, with different activities. These species offer models differing from traditional laboratory experimental animals on the role of melatonin in sperm biology
PB MDPI
LK https://hdl.handle.net/10612/18491
UL https://hdl.handle.net/10612/18491
NO Fernández-Alegre, E., Lacalle, E., Soriano-Úbeda, C., González-Montaña, J. R., Domínguez, J. C., Casao, A., & Martínez-Pastor, F. (2022). Bos taurus and Cervus elaphus as Non-Seasonal/Seasonal Models for the Role of Melatonin Receptors in the Spermatozoon. International Journal of Molecular Sciences, 23(11). https://doi.org/10.3390/IJMS23116284
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 7, 2024