RT info:eu-repo/semantics/article
T1 Caspase 3 Activity and Lipoperoxidative Status in Raw Semen Predict the Outcome of Cryopreservation of Stallion Spermatozoa
A1 Martín Muñoz, Patricia
A1 Ortega Ferrusola, Cristina
A1 Anel López, Luis
A1 del Petre, Chiara
A1 Álvarez García, Mercedes
A1 Paz Cabello, Paulino de
A1 Anel Rodríguez, Luis
A1 Peña Vega, Fernando Juan
A2 Sanidad Animal
K1 Veterinaria
K1 4-HNE
K1 8-ISO-PGF
K1 8-OH guanosine
K1 Cryopreservation
K1 Equids
K1 Flow cytometry
K1 ROS
K1 Sperm
K1 Stallion
AB [EN] Stallion-to-stallion variability in the quality of cryopreserved ejaculates postthaw affects the commercial acceptability of frozen semen and thus is a major constraint for the equine industry. In recent years, the molecular mechanisms associated with sperm damage during cryopreservation have become better understood. Identification of the freezability of the ejaculates before the freezing process is initiated will have a major impact on the equine industry. We studied three markers of oxidative stress in sperm, including 8-iso-PGF2alpha, 8-OH guanosine, and 4-hydroxynonenal (4-HNE); the presence of active caspase 3; and their changes after sperm cryopreservation. Although 4- HNE levels increased after cryopreservation (from 7% to 33%, P < 0.001), 8OH-guanosine and 8-ISO-PGF2alpha levels decreased after cryopreservation (from 130 to 35 arbitrary fluorescence units, P < 0.01, and from 1280 to 1233, P < 0.01, respectively). Postthaw sperm quality was classified as poor, average, or good using the 25th and 75th percentiles of all assays of sperm quality studied (motility, velocity, membrane functionality, and thiol content) as thresholds. Using these values, a sperm postthaw quality index was proposed. Receiver operating characteristic curves and the Youden J statistic were used to investigate the value of the measured parameters in fresh sperm as predictors of potential freezability. Using these techniques, we identified markers of bad freezers (percentages of caspase 3-positive dead sperm [area under the curve (AUC)= 0.820, P < 0.05] and percentages of caspase 3- and 4-HNEpositive sperm [AUC = 0.872, P < 0.05]) and good freezers (percentages of caspase 3-negative live sperm [AUC = 0.815, P < 0.05], percentages of live sperm with high thiol content [AUC = 0.907, P < 0.01], and percentages of 8-ISO-PGF2alphapositive sperm [AUC = 0.900, P < 0.01]. Moreover, we described for the first time the presence of 8-ISO-PGF2alpha in stallion spermatozoa and revealed the importance of considering different markers of oxidative stress.
PB Oxford University Press
SN 0006-3363
LK https://hdl.handle.net/10612/18513
UL https://hdl.handle.net/10612/18513
NO Muñoz, P. M., Ferrusola, C. O., Lopez, L. A., Del Petre, C., Garcia, M. A., Cabello, P. d. P., Anel, L., & Peña, F. J. (2016). Caspase 3 activity and lipoperoxidative status in raw semen predict the outcome of cryopreservation of stallion spermatozoa. Biology of Reproduction, 95(3). https://doi.org/10.1095/BIOLREPROD.116.139444
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 6, 2024