RT info:eu-repo/semantics/article
T1 Secretory IgA as Biomarker for Gastrointestinal Nematodes Natural Infection in Different Breed Sheep
A1 Castilla Gómez de Agüero, Verónica
A1 Valderas García, Elora
A1 González del Palacio, Laura
A1 Giráldez García, Francisco Javier
A1 Balaña Fouce, Rafael
A1 Martínez Valladares, María
A2 Sanidad Animal
K1 Sanidad animal
K1 GIN
K1 Natural infections
K1 IgA
K1 Biomarker
K1 Nasal secretions
K1 Protein disulfide isomerase
K1 L3 somatic antigen
K1 3109 Ciencias Veterinarias
AB [EN] Specific IgA antibody has been shown to play an important role in resistance to gastrointestinal nematode (GIN) infections in sheep, particularly in Teladorsagia circumcincta parasitosis. In some breeds, negative associations have been shown between IgA levels and worm burden in experimentally infected sheep. In the present study, we have studied the relationship between IgA levels in naturally infected sheep (582 ewes in total; 193 younger than one year old and 389 older than one year old) and fecal egg count (FEC) in the Assaf, Castellana, and Churra breeds. ELISA assays were performed to measure IgA levels against the somatic antigen of T. circumcincta third larval stage (L3) and a 203-amino-acid fragment of the protein disulfide isomerase from the same GIN species. A multilevel random intercept model was developed to predict the infection risk according to age or breed. Spearman’s correlation rank was used for statistical analysis. The prediction model showed that breed was not an influential factor in this study, although the Assaf breed could be considered slightly more susceptible than the others. In addition, age affected the infection risk, with the young ewes more susceptible to infection than the adult groups, except for the Castellana breed, whose risk of infection was similar at all ages. The most significant positive association was found between FEC and IgA measured in the nasal secretions of young ewes using both antigens (Rho = 0.5; p = 0.00); the correlation of FEC with IgA in serum was moderately significant (Rho = 0.306; p = 0.00). Comparing both antigens, the protein disulfide isomerase antigen was less reactive than the somatic antigen from L3. In conclusion, under natural conditions, specific IgA against GIN was positively associated with FEC in sheep, with nasal secretions from young animals being the sample where this association is stronger, which, therefore, could be used as a marker of infection in further studies
PB MDPI
LK https://hdl.handle.net/10612/18670
UL https://hdl.handle.net/10612/18670
NO Castilla Gómez de Agüero, V., Valderas-García, E., González del Palacio, L., Giráldez, F. J., Balaña-Fouce, R., & Martínez-Valladares, M. (2023). Secretory IgA as Biomarker for Gastrointestinal Nematodes Natural Infection in Different Breed Sheep. Animals, 13(13). https://doi.org/10.3390/ANI13132189
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 6, 2024