RT info:eu-repo/semantics/article
T1 Immunohistochemical Assessment of Immune Response in the Dermis of Sarcoptes scabiei—Infested Wild Carnivores (Wolf and Fox) and Ruminants (Chamois and Red Deer)
A1 Martínez, Ileana Zorhaya
A1 Oleaga, Álvaro
A1 Sojo, Irene
A1 García Iglesias, María José
A1 Pérez Martínez, Claudia
A1 García Marín, Juan Francisco
A1 Balseiro Morales, Ana María
A2 Sanidad Animal
K1 Sanidad animal
K1 Sarcoptes scabiei
K1 Dermis cellular response
K1 Wolf
K1 Red fox
K1 Chamois
K1 Red deer
K1 Immunohistochemistry
K1 3109 Ciencias Veterinarias
AB [EN] Sarcoptic mange is caused by the mite Sarcoptes scabiei and has been described in several species of domestic and wild mammals. Macroscopic lesions are predominantly hyperkeratotic (type I hypersensitivity) in fox, chamois and deer, but alopecic (type IV hypersensitivity) in wolf and some fox populations. To begin to understand the immune processes underlying these species differences in lesions, we examined skin biopsies from wolves (Canis lupus), foxes (Vulpes vulpes), chamois (Rupicapra rupicapra) and red deer (Cervus elaphus) naturally infested with S. scabiei. Twenty skin samples from five animals per species were used. Sections were immuno-stained with primary antibodies against Iba1 to detect macrophages, lambda chain to detect plasma cells, CD3 to detect T lymphocytes and CD20 to detect B lymphocytes. Skin lesions contained significantly more inflammatory cells in the fox than in the wolf and chamois. Macrophages were the most abundant inflammatory cells in the lesions of all the species studied, suggesting a predominantly innate, non-specific immune response. Lesions from the wolf contained higher proportions of macrophages than the other species, which may reflect a more effective response, leading to alopecic lesions. In red deer, macrophages were significantly more abundant than plasma cells, T lymphocytes and B lymphocytes, which were similarly abundant. The fox proportion of plasma cells was significantly higher than those of T and B lymphocytes. In chamois, T lymphocytes were more abundant than B lymphocytes and plasma cells, although the differences were significant only in the case of macrophages. These results suggest that all the species examined mount a predominantly innate immune response against S. scabiei infestation, while fox and chamois may also mount substantial humoral and cellular immune responses, respectively, with apparently scarce effectiveness that lead to hyperkeratotic lesions.
PB MDPI
LK https://hdl.handle.net/10612/19086
UL https://hdl.handle.net/10612/19086
NO Martínez, I. Z., Oleaga, Á., Sojo, I., García-Iglesias, M. J., Pérez-Martínez, C., García Marín, J. F., & Balseiro, A. (2020). Immunohistochemical assessment of immune response in the dermis of Sarcoptes scabiei—infested wild carnivores (Wolf and fox) and ruminants (chamois and red deer). Animals, 10(7), 1-8. https://doi.org/10.3390/ANI10071146
NO © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/)
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 7, 2024