RT info:eu-repo/semantics/article
T1 Non‐branched β‐1,3‐glucan oligosaccharides trigger immune responses in Arabidopsis
A1 Mélida Martínez, Hugo
A1 Sopeña Torres, Sara
A1 Bacete, Laura
A1 Garrido Arandía, María
A1 Jordá, Lucía
A1 López, Gemma
A1 Muñoz Barrios, Antonio
A1 Pacios, Luis F.
A1 Molina, Antonio
A2 Fisiologia Vegetal
K1 Ingeniería agrícola
K1 Necrotrophic Fungi
K1 Plant Immunity
K1 Cell Wall
K1 Glucan
K1 Chitin
K1 CERK
K1 BAK1
K1 3102 Ingeniería Agrícola
K1 2417.19 Fisiología Vegetal
AB [EN] Fungal cell walls, which are essential for environmental adaptation and host colonization by the fungus,have been evolutionarily selected by plants and animals as a source of microbe-associated molecular patterns(MAMPs) that, upon recognition by host pattern recognition receptors (PRRs), trigger immuneresponses conferring disease resistance. Chito-oligosaccharides [b-1,4-N-acetylglucosamine oligomers,(GlcNAc)n] are the only glycosidic structures from fungal walls that have been well-demonstrated to functionas MAMPs in plants. Perception of (GlcNAc)4–8 by Arabidopsis involves CERK1, LYK4 and LYK5, three ofthe eight members of the LysM PRR family. We found that a glucan-enriched wall fraction from the pathogenicfungus Plectosphaerella cucumerina which was devoid of GlcNAc activated immune responses in Arabidopsiswild-type plants but not in the cerk1 mutant. Using this differential response, we identified thenon-branched 1,3-b-D-(Glc) hexasaccharide as a major fungal MAMP. Recognition of 1,3-b-D-(Glc)6 wasimpaired in cerk1 but not in mutants defective in either each of the LysM PRR family members or in thePRR-co-receptor BAK1. Transcriptomic analyses of Arabidopsis plants treated with 1,3-b-D-(Glc)6 furtherdemonstrated that this fungal MAMP triggers the expression of immunity-associated genes. In silico dockinganalyses with molecular mechanics and solvation energy calculations corroborated that CERK1 can bind1,3-b-D-(Glc)6 at effective concentrations similar to those of (GlcNAc)4. These data support that plants, likeanimals, have selected as MAMPs the linear 1,3-b-D-glucans present in the walls of fungi and oomycetes.Our data also suggest that CERK1 functions as an immune co-receptor for linear 1,3-b-D-glucans in a similarway to its proposed function in the recognition of fungal chito-oligosaccharides and bacterial peptidoglycanMAMPs.
PB Wiley-Blackwell
SN 0960-7412
LK https://hdl.handle.net/10612/20771
UL https://hdl.handle.net/10612/20771
NO Mélida, H., Sopeña-Torres, S., Bacete, L., Garrido-Arandia, M., Jordá, L., López, G., Muñoz-Barrios, A., Pacios, L. F., & Molina, A. (2018). Non-branched β-1,3-glucan oligosaccharides trigger immune responses in Arabidopsis. Plant Journal, 93(1), 34-49. https://doi.org/10.1111/TPJ.13755
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 7, 2024