RT info:eu-repo/semantics/article
T1 The β‐1,3‐glucanosyltransferases (Gels) affect the structure of the rice blast fungal cell wall during appressorium‐mediated plant infection
A1 Samalova, Marketa
A1 Mélida Martínez, Hugo
A1 Vilaplana, Francisco
A1 Bulone, Vincent
A1 Soanes, Darren M.
A1 Talbot, Nicholas J.
A1 Gurr, Sarah J.
A2 Fisiologia Vegetal
K1 Ingeniería agrícola
K1 Beta-Glucans
K1 Cell wall
K1 Glucan Endo-1,3-beta-D-Glucosidase
K1 Gene Deletion
K1 Magnaporthe
K1 Oryza
K1 Plant Diseases
K1 Spores
K1 Fungal
K1 3102 Ingeniería Agrícola
K1 2417.19 Fisiología Vegetal
AB [EN] The fungal wall is pivotal for cell shape and function, and in interfacial protection during host infection and environmental challenge. Here, we provide the first description of the carbohydrate composition and structure of the cell wall of the rice blast fungus Magnaporthe oryzae. We focus on the family of glucan elongation proteins (Gels) and characterize five putative β-1,3-glucan glucanosyltransferases that each carry the Glycoside Hydrolase 72 signature. We generated targeted deletion mutants of all Gel isoforms, that is, the GH72+, which carry a putative carbohydrate-binding module, and the GH72− Gels, without this motif. We reveal that M. oryzae GH72+ GELs are expressed in spores and during both infective and vegetative growth, but each individual Gel enzymes are dispensable for pathogenicity. Further, we demonstrated that a Δgel1Δgel3Δgel4 null mutant has a modified cell wall in which 1,3-glucans have a higher degree of polymerization and are less branched than the wild-type strain. The mutant showed significant differences in global patterns of gene expression, a hyper-branching phenotype and no sporulation, and thus was unable to cause rice blast lesions (except via wounded tissues). We conclude that Gel proteins play significant roles in structural modification of the fungal cell wall during appressorium-mediated plant infection.
PB Wiley-Blackwell
SN 1462-5814
LK https://hdl.handle.net/10612/20796
UL https://hdl.handle.net/10612/20796
NO Samalova, M., Mélida, H., Vilaplana, F., Bulone, V., Soanes, D. M., Talbot, N. J., & Gurr, S. J. (2017). The β-1,3-glucanosyltransferases (Gels) affect the structure of the rice blast fungal cell wall during appressorium-mediated plant infection. Cellular Microbiology, 19(3). https://doi.org/10.1111/CMI.12659
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 8, 2024