RT info:eu-repo/semantics/article
T1 Volatile Organic Compound Chamber: A Novel Technology for Microbiological Volatile Interaction Assays
A1 Álvarez García, Samuel
A1 Mayo Prieto, Sara
A1 Carro-Huerga, Guzmán
A1 Rodríguez González, Álvaro
A1 González López, Óscar
A1 Gutiérrez Martín, Santiago
A1 Casquero Luelmo, Pedro Antonio
A2 Produccion Vegetal
K1 Ingeniería agrícola
K1 Volatile organic compounds
K1 VOCs
K1 Trichodiene
K1 Squalene
K1 Trichoderma
K1 Fusarium
K1 Rhizoctonia
K1 Biocontrol
K1 Biological control
AB [EN] The interest in the study of microbiological interactions mediated by volatile organic compounds (VOCs) has steadily increased in the last few years. Nevertheless, most assays still rely on the use of non-specific materials. We present a new tool, the volatile organic compound chamber (VOC chamber), specifically designed to perform these experiments. The novel devices were tested using four Trichoderma strains against Fusarium oxysporum and Rhizoctonia solani. We demonstrate that VOC chambers provide higher sensitivity and selectivity between treatments and higher homogeneity of results than the traditional method. VOC chambers are also able to test both vented and non-vented conditions. We prove that ventilation plays a very important role regarding volatile interactions, up to the point that some growth-inhibitory effects observed in closed environments switch to promoting ones when tested in vented conditions. This promoting activity seems to be related to the accumulation of squalene by T. harzianum. The VOC chambers proved to be an easy, homogeneous, flexible, and repeatable method, able to better select microorganisms with high biocontrol activity and to guide the future identification of new bioactive VOCs and their role in microbial interactions
PB MDPI
LK https://hdl.handle.net/10612/21281
UL https://hdl.handle.net/10612/21281
NO Álvarez-García, S., Mayo-Prieto, S., Carro-Huerga, G., Rodríguez-González, Á., González-López, Ó., Gutiérrez, S., & Casquero, P. A. (2021). Volatile organic compound chamber: A novel technology for microbiological volatile interaction assays. Journal of Fungi, 7(4). https://doi.org/10.3390/JOF7040248
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 7, 2024