RT info:eu-repo/semantics/article
T1 Effects of Trichothecene Production on the Plant Defense Response and Fungal Physiology: Overexpression of the Trichoderma arundinaceum            tri4            Gene in T. harzianum
A1 Cardoza, Rosa E.
A1 McCormick, Susan P.
A1 Gómez Malmierca, Mónica
A1 Rodríguez Olivera, Elías
A1 Alexander, N. J.
A1 Monte Vázquez, Enrique
A1 Gutiérrez Martín, Santiago
A2 Microbiologia
K1 Biología
K1 Trichothecene
K1 Plant defense
K1 Fungal physiology
K1 Trichoderma arundinaceum tri4 gene
K1 T. harzianum
AB [EN] Trichothecenes are fungal sesquiterpenoid compounds, the majority of which have phytotoxic activity. They contaminate food and feed stocks, resulting in potential harm to animals and human beings. Trichoderma brevicompactum and T. arundinaceum produce trichodermin and harzianum A (HA), respectively, two trichothecenes that show different bioactive properties. Both compounds have remarkable antibiotic and cytotoxic activities, but in addition, trichodermin is highly phytotoxic, whileHAlacks this activity when analyzed in vivo. Analysis of Fusarium trichothecene intermediates led to the conclusion that most of them, with the exception of the hydrocarbon precursor trichodiene (TD), have a detectable phytotoxic activity which is not directly related to the structural complexity of the intermediate. In the present work, theHAintermediate 12,13-epoxytrichothec-9-ene (EPT) was produced by expression of the T. arundinaceum tri4 gene in a transgenic T. harzianum strain that already produces TD after transformation with the T. arundinaceum tri5 gene. Purified EPT did not show antifungal or phytotoxic activity, while purified HA showed both antifungal and phytotoxic activities. However, the use of the transgenic T. harzianum tri4 strain induced a downregulation of defense-related genes in tomato plants and also downregulated plant genes involved in fungal root colonization. The production of EPT by the transgenic tri4 strain raised levels of erg1 expression and reduced squalene accumulation while not affecting levels of ergosterol. Together, these results indicate the complex interactions among trichothecene intermediates, fungal antagonists, and host plants
PB American Society for Microbiology
SN 0099-2240
LK https://hdl.handle.net/10612/21647
UL https://hdl.handle.net/10612/21647
NO Cardoza, McCormick, Malmierca, Olivera, Alexander, Monte, & Gutiérrez. (2015). Effects of trichothecene production on the plant defense response and fungal physiology: Overexpression of the Trichoderma arundinaceum tri4 gene in T. harzianum. Applied and Environmental Microbiology, 81(18), 6355-6366. https://doi.org/10.1128/AEM.01626-15
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 7, 2024