RT info:eu-repo/semantics/article
T1 Trichodiene Production in aTrichoderma harzianum erg1-Silenced Strain Provides Evidence of the Importance of the Sterol Biosynthetic Pathway in Inducing Plant Defense-Related Gene Expression
A1 Gómez Malmierca, Mónica
A1 McCormick, Susan P.
A1 Cardoza, Rosa E.
A1 Monte Vázquez, Enrique
A1 Alexander, N. J.
A1 Gutiérrez Martín, Santiago
A2 Microbiologia
K1 Biología
K1 Trichodiene
K1 Trichoderma harzianum
K1 Sterol Biosynthetic Pathway
K1 Plant defense
AB [EN] Trichoderma species are often used as biocontrol agents against plant-pathogenic fungi. A complex molecular interaction occurs among the biocontrol agent, the antagonistic fungus, and the plant. Terpenes and sterols produced by the biocontrol fungus have been found to affect gene expression in both the antagonistic fungus and the plant. The terpene trichodiene (TD) elicits the expression of genes related to tomato defense and to Botrytis virulence. We show here that TD itself is able to induce the expression of Botrytis genes involved in the synthesis of botrydial (BOT) and also induces terpene gene expression in Trichoderma spp. The terpene ergosterol, in addition to its role as a structural component of the fungal cell membranes, acts as an elicitor of defense response in plants. In the present work, using a transformant of T. harzianum, which is silenced in the erg1 gene and accumulates high levels of squalene, we show that this ergosterol precursor also acts as an important elicitor molecule of tomato defense-related genes and induces Botrytis genes involved in BOT biosynthesis, in both cases, in a concentration-dependent manner. Our data emphasize the importance of a balance of squalene and ergosterol in fungal interactions as well as in the biocontrol activity of Trichoderma spp
PB American Phytopathological Society
SN 0894-0282
LK https://hdl.handle.net/10612/21649
UL https://hdl.handle.net/10612/21649
NO Malmierca, McCormick, Cardoza, Monte, Alexander, & Gutiérrez. (2015). Trichodiene Production in a Trichoderma harzianum erg1-Silenced Strain Provides Evidence of the Importance of the Sterol Biosynthetic Pathway in Inducing Plant Defense-Related Gene Expression. Molecular Plant-Microbe Interactions, 28(11), 1181-1197. https://doi.org/10.1094/MPMI-06-15-0127-R
DS BULERIA. Repositorio Institucional de la Universidad de León
RD Jul 7, 2024