dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Rodríguez Cadenas, Fausto | |
dc.contributor.author | Carbajal González, María Teresa | |
dc.contributor.author | Fregeneda Grandes, Juan Miguel | |
dc.contributor.author | Aller Gancedo, José Miguel | |
dc.contributor.author | Rojo Vázquez, Francisco Antonio | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2010-02-15 | |
dc.date.accessioned | 2019-04-17T17:22:25Z | |
dc.date.available | 2019-04-17T17:22:25Z | |
dc.date.issued | 2019-04-17 | |
dc.identifier.citation | Veterinary Immunology and Immunopathology, 2010, vol. 133, n. 2-4 | es_ES |
dc.identifier.other | https://doi.org/10.1016/j.vetimm.2009.07.004 | es_ES |
dc.identifier.uri | http://hdl.handle.net/10612/10369 | |
dc.description | P. 109-116 | es_ES |
dc.description.abstract | In this work the clinical evolution and the specific serum IgG and IgE antibody responses in
sheep after primary (n = 10) and secondary (n = 4) experimental challenges with the
mange mite Sarcoptes scabiei var. ovis were studied. The primary infection was
characterized by the development of mange lesions in all sheep, a detection of live S.
scabiei mites in 70% skin scrapings taken in week 10 post-challenge (PC), strongly raised
and sustained specific IgG levels and a more moderate but continuous rise in specific IgE
levels. Seroconversion was detected for IgG and IgE by ELISA in 90% and 60% of the sheep in
week 8 PC, respectively. By Western-blotting (WB), ten IgG-reactive bands (36–120 kDa)
and four IgE-reactive bands (90–180 kDa) were observed in week 8 PC. Following the
secondary challenge the ewes developed a smaller area of mange lesion than that seen
following primary challenge and live S. scabiei mites were not detected in skin scrapings
collected in week 8 PC, suggesting that sheep had developed immunity to re-infection.
Compared to primary infection, the specific IgG secondary antibody levels were transient,
but in contrast there was an anamnestic IgE response, resulting in an elicitation of specific
serum IgE levels in week 2 PC significantly higher than those demonstrated after primary
infection. WB analysis revealed one additional IgG-reactive band (180 kDa) and no
additional IgE-reactive bands. Determining the immunodiagnostic or vaccination value of
the IgG-reactive antigens and IgE-reactive allergens detected requires further studies. | es_ES |
dc.language | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.subject | Sanidad animal | es_ES |
dc.subject | Veterinaria | es_ES |
dc.subject.other | Inmunoglobulinas | es_ES |
dc.subject.other | Sarna sarcóptica | es_ES |
dc.subject.other | Ganado ovino | es_ES |
dc.title | Clinical evaluation and antibody responses in sheep after primary and secondary experimental challenges with the mange mite Sarcoptes scabiei var. ovis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.peerreviewed | SI | es_ES |