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dc.contributor | Facultad de Ciencias Biologicas y Ambientales | es_ES |
dc.contributor.author | Castro González, David | |
dc.contributor.author | Álvarez García, Mercedes | |
dc.contributor.author | Muro, J. | |
dc.contributor.author | Esteso, Milagros | |
dc.contributor.author | Paz Cabello, Paulino de | |
dc.contributor.author | Anel Rodríguez, Luis | |
dc.contributor.author | Martínez Pastor, Felipe | |
dc.contributor.other | Biologia Celular | es_ES |
dc.date | 2010-04 | |
dc.date.accessioned | 2019-05-13T17:38:09Z | |
dc.date.available | 2019-05-13T17:38:09Z | |
dc.date.issued | 2019-05-13 | |
dc.identifier.citation | Reproduction in Domestic Animals, 2010, vol. 45, n. 2 | es_ES |
dc.identifier.other | https://onlinelibrary.wiley.com/doi/full/10.1111/j.1439-0531.2008.01333.x | es_ES |
dc.identifier.uri | http://hdl.handle.net/10612/10749 | |
dc.description | P. 363-367 | es_ES |
dc.description.abstract | To try new acrosomal probes for assessing ram spermatozoa, we compared the LysoSensor™ probe, which labels acidic organelles, with the frequently used peanut agglutinin acrosomal probe (PNA‐PE; phycoerythrin as fluorescent moiety). The previous microscopic observations showed a lack of relationship of LysoSensor™ with acrosomal status. Semen was obtained from five rams and frozen in four pools. Each pool was analysed carrying out a triple staining propidium ioide/PNA‐PE/LysoSensor™ Green DND‐189 to test acrosome labelling, and a double staining SYBR‐14/PI, to assess sperm viability. Stained samples were analysed by flow cytometry. All measurements were replicated. Data were processed using agreement and repeatability tests. LysoSensor™ labelling did not agree with PNA (mean of differences: 30.8%; coefficient of agreement: 22.6%), confirming microscopic observations. Nevertheless, when LysoSensor™ was compared with SYBR‐14/PI, the agreement was high (mean of differences: −0.05%; coefficient of agreement: 5.07%). Repeatability of both methods was high and similar. LysoSensor™ did not seem to specifically stain the acrosome, but it may accumulate in the cytoplasm and label viable spermatozoa. Therefore, LysoSensor™ might not be used as an acrosomal probe in ram spermatozoa, but it could be used in other kind of studies, taking advantage of its pH sensitivity. | es_ES |
dc.language | eng | es_ES |
dc.publisher | John Wiley & Sons | es_ES |
dc.subject | Veterinaria | es_ES |
dc.subject.other | Criopreservación | es_ES |
dc.subject.other | Esperma | es_ES |
dc.subject.other | Ganado ovino | es_ES |
dc.title | The Acidic Probe LysoSensor™ is not Useful for Acrosome Evaluation of Cryopreserved Ram Spermatozoa | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.description.peerreviewed | SI | es_ES |
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