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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | González Fernández, Alba | |
dc.contributor.author | Gutiérrez Martín, César Bernardo | |
dc.contributor.author | Petrocchi Rilo, Maximo | |
dc.contributor.author | Pérez Fernández, Esther | |
dc.contributor.author | Miguélez Pérez, Ruben | |
dc.contributor.author | Frandoloso, Rafael | |
dc.contributor.author | Martínez Martínez, Sonia | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2023 | |
dc.date.accessioned | 2023-03-03T11:07:23Z | |
dc.date.available | 2023-03-03T11:07:23Z | |
dc.identifier.citation | Fernández, A. G., Martín, C. B. G., Rilo, M. P., Fernández, E. P., Pérez, R. M., Frandoloso, R., & Martínez, S. M. (2023). Phylogenetic study and comparison of different TbpB obtained from Glaesserella parasuis present in Spanish clinical isolates. Research in Veterinary Science, 157, 35-39. https://doi.org/10.1016/j.rvsc.2023.02.003 | es_ES |
dc.identifier.issn | 0034-5288 | |
dc.identifier.uri | http://hdl.handle.net/10612/15704 | |
dc.description.abstract | [EN] Glaesserella parasuis (Gp) is the etiological agent of Glässer's disease (GD), which causes important economic losses for the pig intensive production worldwide. This organism uses a smart protein-based receptor to acquire specifically iron from the porcine transferrin. This surface receptor consists of transferrin-binding protein A (TbpA) and transferrin-binding protein B (TbpB). TbpB has been considered the most promising antigen to formulate a based-protein vaccine with broad-spectrum of protection against GD. The purpose of our study was to determine the capsular diversity of Gp clinical isolates collected in different Spanish regions between 2018 and 2021. A total of 68 Gp isolates were recovered from porcine respiratory or systemic samples. A species-specific PCR based on tbpA gene, followed by multiplex PCR for typing Gp isolates were performed. Serovars 5, 10, 2, 4 and 1 were the most prevalent and involved almost 84% of isolates. TbpB amino acid sequences from 59 of these isolates were analyzed, and a total of ten clades could be established. All of them showed a wide diversity with respect to capsular type, anatomical isolation site and geographical origin, with minor exceptions. Regardless of the serovars, the in silico analysis of TbpB sequences revealed that a vaccine based on a TbpB recombinant protein could potentially prevent Glässer's disease outbreaks in Spain. | es_ES |
dc.language | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Sanidad animal | es_ES |
dc.subject.other | Glaesserella parasuis | es_ES |
dc.subject.other | Glässer's disease | es_ES |
dc.subject.other | Serotyping | es_ES |
dc.subject.other | Sequencing | es_ES |
dc.subject.other | Phylogenetic relationships | es_ES |
dc.title | Phylogenetic study and comparison of different TbpB obtained from Glaesserella parasuis present in Spanish clinical isolates | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1016/j.rvsc.2023.02.003 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/10.13039/501100011033 | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.journal.title | Research in Veterinary Science | es_ES |
dc.volume.number | 157 | es_ES |
dc.page.initial | 35 | es_ES |
dc.page.final | 39 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
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