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Título
Characterization and expression of the arginine biosynthesis gene cluster of Streptomyces clavuligerus
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Journal of Molecular Microbiology and Biotechnology
Número de la revista
4
Datos de la obra
Rodríguez-García, A., De La Fuente, A., Pérez-Redondo, R., Martín, J. F. & Liras, P. (2000). Characterization and expression of the arginine biosynthesis gene cluster of Streptomyces clavuligerus. Journal of Molecular Microbiology and Biotechnology, 2(4), 543-550
Editor
Karger
Fecha
2000-04-14
ISSN
1464-1801
Resumo
[EN] A cluster of genes argCJBDRGH containing most of the arginine biosynthesis genes has been found in Streptomyces clavuligerus after sequencing a 8.3 kb DNA region containing overlapping sequences of two DNA fragments known to contain arginine biosynthesis genes. Subcloning, complementation of E. coli arginine auxotrophic strains and enzymatic assays confirmed the identity of each gene. S1 nuclease mapping studies and Northern hybridization analysis revealed the formation of two large transcripts corresponding to argCJBDR and argGH. The amount of each of these mRNAs is 10 to 44 times higher in a S. clavuligerus argR-disrupted mutant than in the wild type confirming the existence of an ArgR-mediated control of arginine biosynthesis gene expression. A low level constitutive monocistronic transcript of argR was observed in S. clavuligerus cells. Most of the argGH transcript initiating at an adenine 29 nt upstream of the argG initiation codon appears to stop at a termination stem and loop structure present downstream of the argG gene
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Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/Plan Nacional de Investigación Científica y Desarrollo Tecnológico/BIO96-0827/ES/Ingeniería metabólica de streptomyces clavuligerus para la eliminación de los mecanismos de control que limitan la biosíntesis del ácido clavulánico y de sus precursores
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