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Título
Towards the design of universal immunosurfaces for SPR-based assays: A review
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
TrAC Trends in Analytical Chemistry
Datos de la obra
MaurizE.,García-FernándezM.C.,LechugaL.M..Towardsthe designofuniversal immunosurfacesforSPR-basedassays:A review.TrAC-TrendsinAnalyticalChemistry,(2016).79.:191-.10.1016/j.trac.2016.02.006,
Editor
Elsevier
Fecha
2016
ISSN
0165-9936
Zusammenfassung
Surface biofunctionalization, including chemical activation and attachment of the bioreceptor, is an essential step to provide reliable detection of biomolecular binding events monitored by Surface Plasmon Resonance (SPR), the most employed optical biosensor, and other biosensor techniques. Recent progress in the area of immobilization procedures are aimed at producing reproducible interfacial surfaces that enable the sensitive and specific recognition of the analyte. Antibodies are still the most employed bioreceptors for SPR assays. A wide range of strategies have been proposed to maximize the SPR immunosensor performance by controlling the stability and orientation of the immobilized antibody. This article reviews the most recent advancements in random and oriented antibody immobilization approaches for SPR biosensing applications, with a special focus on the research that have been done to find universal linkers, which can allow the use of the same functionalized surface for different applications.
Materia
Palabras clave
Peer review
SI
URI
DOI
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