Compartir
Título
Metabolic switches and adaptations deduced from the proteomes of Streptomyces coelicolor wild type and phoP mutant grown in batch culture
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Molecular & Cellular Proteomics
Número de la revista
2
Datos de la obra
Thomas, L., Hodgson, D. A., Wentzel, A., Nieselt, K., Ellingsen, T. E., Moore, J., Morrissey, E. R., Legaie, R., Wohlleben, W., Rodríguez-García, A., Martín, J. F., Burroughs, N. J., Wellington, E. M. H., & Smith, M. C. M. (2012). Metabolic switches and adaptations deduced from the proteomes of Streptomyces coelicolor wild type and phoP mutant grown in batch culture Molecular and Cellular Proteomics, 11(2), Article eM111.013797. https://doi.org/10.1074/MCP.M111.013797
Editor
American Society for Biochemistry and Molecular Biology
Fecha
2012-02-01
ISSN
1535-9476
Resumo
[EN] Bacteria in the genus Streptomyces are soil-dwelling oligotrophs and important producers of secondary metabolites. Previously, we showed that global messenger RNA expression was subject to a series of metabolic and regulatory switches during the lifetime of a fermentor batch culture of Streptomyces coelicolor M145. Here we analyze the proteome from eight time points from the same fermentor culture and, because phosphate availability is an important regulator of secondary metabolite production, compare this to the proteome of a similar time course from an S. coelicolor mutant, INB201 (ΔphoP), defective in the control of phosphate utilization. The proteomes provide a detailed view of enzymes involved in central carbon and nitrogen metabolism. Trends in protein expression over the time courses were deduced from a protein abundance index, which also revealed the importance of stress pathway proteins in both cultures. As expected, the ΔphoP mutant was deficient in expression of PhoP-dependent genes, and several putatively compensatory metabolic and regulatory pathways for phosphate scavenging were detected. Notably there is a succession of switches that coordinately induce the production of enzymes for five different secondary metabolite biosynthesis pathways over the course of the batch cultures
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/BBSRC/BB/F003439/1
- info:eu-repo/grantAgreement/EU//GEN2006-27745-E/SYS
- info:eu-repo/grantAgreement/RCN//181840/130
- info:eu-repo/grantAgreement/EC/FP7/235447/EU
URI
DOI
Versión del editor
Aparece en las colecciones
- Untitled [5133]
Arquivos deste item
Tamaño:
4.238
xmlui.dri2xhtml.METS-1.0.size-megabytes
Formato:
Adobe PDF