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Título
Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Nucleic Acids Research
Número de la revista
3
Cita Bibliográfica
Sola-Landa, A., Rodríguez-García, A., Amin, R., Wohlleben, W., & Martín, J. F. (2013). Competition between the GlnR and PhoP regulators for the glnA and amtB promoters in Streptomyces coelicolor. Nucleic Acids Research, 41(3), 1767-1782. https://doi.org/10.1093/NAR/GKS1203
Editorial
Oxford University Press
Fecha
2013-02-01
ISSN
0305-1048
Resumen
[EN] Interaction of regulatory networks is a subject of great interest in systems biology of bacteria. Phosphate control of metabolism in Streptomyces is mediated by the two-component system PhoR-PhoP. Similarly, the utilization of different nitrogen sources is controlled by the regulator GlnR. Transcriptomic and biochemical analysis revealed that glnA (encoding a glutamine synthetase), glnR and other nitrogen metabolism genes are under PhoP control. DNA-binding experiments showed that PhoP binds to other nitrogen-regulated genes (SCO0255, SCO01863 and ureA). Using the glnA promoter as model, we observed that PhoP and GlnR compete for binding to the same promoter region, showing GlnR a higher affinity. Using a total of 14 GlnR-binding sites (50 direct repeat units) we established two information-based models that describe the GlnR box as consisting of two 11-nt direct repeats each with clear differences to PHO box. DNA-binding studies with different mutant sequences of glnA promoter revealed that the sequence recognized by GlnR is found in the coding strand whereas that recognized by PhoP is overlapping in the non-coding strand. In amtB promoter PhoP and GlnR boxes are not totally overlapping and both proteins bind simultaneously. PhoP control of nitrogen metabolism genes helps to balance the cellular P/N equilibrium
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/EU//GEN2006-27745-E/SYS
- info:eu-repo/grantAgreement/CICYT//BIO2010-16094/ES
URI
DOI
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