Compartir
Título
LAL regulators SCO0877 and SCO7173 as pleiotropic modulators of phosphate starvation response and actinorhodin biosynthesis in Streptomyces coelicolor
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
PLoS ONE
Número de la revista
2
Cita Bibliográfica
Guerra, S. M., Rodríguez-García, A., Santos-Aberturas, J., Vicente, C. M., Payero, T. D., Martín, J. F., & Aparicio, J. F. (2012). LAL regulators SCO0877 and SCO7173 as pleiotropic modulators of phosphate starvation response and actinorhodin biosynthesis in Streptomyces coelicolor. PLoS ONE, 7(2), Article e31475. https://doi.org/10.1371/JOURNAL.PONE.0031475
Editorial
Public Library of Science
Fecha
2012-02-20
Resumen
[EN] LAL regulators (Large ATP-binding regulators of the LuxR family) constitute a poorly studied family of transcriptional regulators. Several regulators of this class have been identified in antibiotic and other secondary metabolite gene clusters from actinomycetes, thus they have been considered pathway-specific regulators. In this study we have obtained two disruption mutants of LAL genes from S. coelicolor (Δ0877 and Δ7173). Both mutants were deficient in the production of the polyketide antibiotic actinorhodin, and antibiotic production was restored upon gene complementation of the mutants. The use of whole-genome DNA microarrays and quantitative PCRs enabled the analysis of the transcriptome of both mutants in comparison with the wild type. Our results indicate that the LAL regulators under study act globally affecting various cellular processes, and amongst them the phosphate starvation response and the biosynthesis of the blue-pigmented antibiotic actinorhodin. Both regulators act as negative modulators of the expression of the two-component phoRP system and as positive regulators of actinorhodin biosynthesis. To our knowledge this is the first characterization of LAL regulators with wide implications in Streptomyces metabolism
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/MICINN//BIO2007-67585
URI
DOI
Versión del editor
Aparece en las colecciones
- Artículos [5134]
Ficheros en el ítem
Tamaño:
444.5
xmlui.dri2xhtml.METS-1.0.size-kilobytes
Formato:
Adobe PDF