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dc.contributor | Facultad de Ciencias Biologicas y Ambientales | es_ES |
dc.contributor.author | Pérez Redondo, Rosario | |
dc.contributor.author | Rodríguez García, Antonio | |
dc.contributor.author | Botas Muñoz, Alma María | |
dc.contributor.author | Santamarta, Irene | |
dc.contributor.author | Martín Martín, Juan Francisco | |
dc.contributor.author | Liras Padín, Paloma | |
dc.contributor.other | Microbiologia | es_ES |
dc.date | 2012-03-05 | |
dc.date.accessioned | 2024-02-01T08:07:32Z | |
dc.date.available | 2024-02-01T08:07:32Z | |
dc.identifier.citation | Pérez-Redondo, R., Rodríguez-García, A., Botas, A., Santamarta, I., Martín, J. F., & Liras, P. (2012). ArgR of Streptomyces coelicolor is a versatile regulator. PLoS ONE, 7(3), Article e32697. https://doi.org/10.1371/JOURNAL.PONE.0032697 | es_ES |
dc.identifier.other | https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0032697 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/17979 | |
dc.description.abstract | [EN] ArgR is the regulator of arginine biosynthesis genes in Streptomyces species. Transcriptomic comparison by microarrays has been made between Streptomyces coelicolor M145 and its mutant S. coelicolor ΔargR under control, unsupplemented conditions, and in the presence of arginine. Expression of 459 genes was different in transcriptomic assays, but only 27 genes were affected by arginine supplementation. Arginine and pyrimidine biosynthesis genes were derepressed by the lack of ArgR, while no strong effect on expression resulted on arginine supplementation. Several nitrogen metabolism genes expression as glnK, glnA and glnII, were downregulated in S. coelicolor ΔargR. In addition, downregulation of genes for the yellow type I polyketide CPK antibiotic and for the antibiotic regulatory genes afsS and scbR was observed. The transcriptomic data were validated by either reverse transcription-PCR, expression of the gene-promoter coupled to the luciferase gene, proteomic or by electrophoresis mobility shift assay (EMSA) using pure Strep-tagged ArgR. Two ARG-boxes in the arginine operon genes suggest that these genes are more tightly controlled. Other genes, including genes encoding regulatory proteins, possess a DNA sequence formed by a single ARG-box which responds to ArgR, as validated by EMSA | es_ES |
dc.language | eng | es_ES |
dc.publisher | Public Library of Science | es_ES |
dc.subject | Biología | es_ES |
dc.subject.other | Streptomyces coelicolor | es_ES |
dc.subject.other | ArgR | es_ES |
dc.subject.other | EMSA | es_ES |
dc.subject.other | Transcriptomics | es_ES |
dc.subject.other | Antibotics biosynthesis regulation | es_ES |
dc.subject.other | Arginine regulation | es_ES |
dc.title | ArgR of Streptomyces coelicolor is a versatile regulator | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1371/journal.pone.0032697 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/CICYT//BIO2009-09820 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/CICYT//GEN2003-20245 | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/EU//LSHM-CT-2004-005224/ACTINOGEN | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1932-6203 | |
dc.journal.title | PLoS ONE | es_ES |
dc.volume.number | 7 | es_ES |
dc.issue.number | 3 | es_ES |
dc.page.initial | 32697 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 2414.02 Fisiología Bacteriana | es_ES |
dc.subject.unesco | 2415.01 Biología Molecular de Microorganismos | es_ES |
dc.description.project | This work was supported by Grants from the Spanish Comisión Interministerial de Ciencia y Tecnología GEN2003-20245, BIO2009-09820, and by the European Project LSHM-CT-2004-005224. AB received a fellowship from the Ministry of Science and Innovation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | es_ES |
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