Mostrar el registro sencillo del ítem
dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Puente Fernández, Héctor | |
dc.contributor.author | Díaz, Iván | |
dc.contributor.author | Argüello Rodríguez, Héctor | |
dc.contributor.author | Mencía Ares, Óscar | |
dc.contributor.author | Gómez García, Manuel | |
dc.contributor.author | Pérez Pérez, Lucía | |
dc.contributor.author | Vega, Clara | |
dc.contributor.author | Cortey, Martí | |
dc.contributor.author | Martín, Margarita | |
dc.contributor.author | Rubio Nistal, Pedro Miguel | |
dc.contributor.author | Carvajal Urueña, Ana María | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2022 | |
dc.date.accessioned | 2024-02-02T07:00:43Z | |
dc.date.available | 2024-02-02T07:00:43Z | |
dc.identifier.citation | Puente, H., Díaz, I., Arguello, H., Mencía-Ares, Ó., Gómez-García, M., Pérez-Pérez, L., Vega, C., Cortey, M., Martín, M., Rubio, P., & Carvajal, A. (2022). Characterization and cross-protection of experimental infections with SeCoV and two PEDV variants. Transboundary and Emerging Diseases, 69(6), 3225-3237. https://doi.org/10.1111/TBED.14674 | es_ES |
dc.identifier.issn | 1865-1674 | |
dc.identifier.other | https://onlinelibrary.wiley.com/doi/10.1111/tbed.14674 | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/18006 | |
dc.description.abstract | [EN] The aim of this study was to characterize the infection of weaned pigs with swine enteric coronavirus (SeCoV) – a chimeric virus most likely originated from a recombination event between porcine epidemic diarrhoea virus (PEDV) and transmissible gastroenteritis virus, or its mutant porcine respiratory coronavirus – and two PEDV G1b variants, including a recently described recombinant PEDV-SeCoV (rPEDV-SeCoV), as well as to determine the degree of cross-protection achieved against the rPEDV-SeCoV. For this purpose, forty-eight 4-week-old weaned pigs were randomly allocated into four groups of 12 animals. Piglets within each group were primary inoculated with one of the investigated viral strains (B: PEDV; C: SeCoV and D: rPEDV-SeCoV) or mock-inoculated (A), and exposed to rPEDV-SeCOV at day 20 post-infection; thus, group A was primary challenged (-/rPEDV-SeCoV), groups B and C were subjected to a heterologous re-challenge (PEDV/rPEDV-SeCoV and SeCoV/rPEDV-SeCoV, respectively), and group D to a homologous re-challenge (rPEDV-SeCoV/rPEDV-SeCoV), Clinical signs, viral shedding, microscopic lesions and specific humoral and cellular immune responses (IgG, IgA, neutralizing antibodies and IgA and IFN-γ-secreting cells) were monitored. After primo-infection, all three viral strains induced an undistinguishable mild-to-moderate clinical disease with diarrhoea as the main sign and villus shortening lesions in the small intestine. In homologous re-challenged pigs, no clinical signs or lesions were observed, and viral shedding was only detected in a single animal. This fact may be explained by the significant high level of rPEDV-SeCoV-specific neutralizing antibodies found in these pigs before the challenge. In contrast, prior exposure to a different PEDV G1b variant or SeCoV only provided partial cross-protection, allowing rPEDV-SeCoV replication and shedding in faeces | es_ES |
dc.language | eng | es_ES |
dc.publisher | Wiley | es_ES |
dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
dc.subject | Sanidad animal | es_ES |
dc.subject | Veterinaria | es_ES |
dc.subject.other | Immunity | es_ES |
dc.subject.other | Porcine epidemic diarrhoea virus | es_ES |
dc.subject.other | Recombinant | es_ES |
dc.subject.other | Swine coronavirus | es_ES |
dc.subject.other | Swine enteric coronavirus | es_ES |
dc.title | Characterization and cross‐protection of experimental infections with SeCoV and two PEDV variants | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1111/TBED.14674 | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MECD/Programa Estatal de Promoción del Talento y su Empleabilidad/ FPU16/03485/ES/ / / | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1865-1682 | |
dc.journal.title | Transboundary and Emerging Diseases | es_ES |
dc.volume.number | 69 | es_ES |
dc.issue.number | 6 | es_ES |
dc.page.initial | 3225 | es_ES |
dc.page.final | 3237 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/acceptedVersion | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.description.project | This work was supported by the program of the National Instituteof Agricultural and Food Research and Technology (INIA projectE-RTA2015-0003-C02-01 and E-RTA2015-0003-C02-02) of SpanishGovernment.H.Puente,O.Mencía-Ares,L.Pérez-Pérez,M.CorteyandH.ArgüelloweresupportedbySpanishGovernment(FPU17/00466,FPU16/03485, PRE2020-093762, RYC-2015-1715-4 and BEAGAL-18-106, respectively) and M. Gómez-García by Junta de Castilla, &León(LE131-18) | es_ES |
Ficheros en el ítem
Este ítem aparece en la(s) siguiente(s) colección(ones)
-
Artículos [5398]