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Título
Molecular Identification and Acid Stress Response of an Acidithiobacillus thiooxidans Strain Isolated from Rio Tinto (Spain)
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
International Journal of Molecular Sciences
Número de la revista
17
Datos de la obra
Ibáñez, A., Barreiro, C., Diez-Galán, A., Cobos, R., Calvo-Peña, C., & Coque, J. J. R. (2023). Molecular Identification and Acid Stress Response of an Acidithiobacillus thiooxidans Strain Isolated from Rio Tinto (Spain). International Journal of Molecular Sciences, 24(17). https://doi.org/10.3390/IJMS241713391
Editor
MDPI
Fecha
2023-08-29
Resumo
[EN] Acidithiobacillus thiooxidans is of paramount importance in the development of biomining technologies. Being widely recognized as an extreme acidophile, extensive research has been dedicated to understanding its significant role in the extraction of several ores in recent years. However, there still exist significant molecular uncertainties surrounding this species. This study focuses on developing a taxonomic assignment method based on the sequencing of the 16S-5S rRNA cluster, along with a qPCR-based technology enabling precise growth determination. Additionally, an approach to understanding its response to acid stress is explored through RT-PCR and MALDITOF analysis. Our findings indicate that when subjected to pH levels below 1, the cell inhibits central (carbon fixation and metabolism) and energy (sulfur metabolism) metabolism, as well as chaperone synthesis, suggesting a potential cellular collapse. Nevertheless, the secretion of ammonia is enhanced to raise the environmental pH, while fatty acid synthesis is upregulated to reinforce the cell membrane.
Materia
Palabras clave
Peer review
SI
URI
DOI
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