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Título
Progesterone stimulates the long-distance migration of capacitated ram spermatozoa through viscous media under geotactic condition
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Theriogenology
Datos de la obra
Martinez-Rodriguez, C., Anel-Lopez, L., Alvarez, M., Ortega-Ferrusola, C., Boixo, J. C., Pena, F. J., ... & de Paz, P. (2018). Progesterone stimulates the long-distance migration of capacitated ram spermatozoa through viscous media under geotactic condition. Theriogenology, 118, 7-15.
Editor
Elsevier Inc.
Fecha
2018
ISSN
0093-691X
Abstract
[EN] Forward progressive motility of spermatozoa is an essential prerequisite for reproductive success, and sperm navigation is assisted by guidance mechanisms that may depend on micro environmental factors. In the present study, we performed an integrated analysis of long distance ram sperm migration in vitro that combined two environmental factors (10 μM progesterone and a geotactic effect) and the physiological status of the cells (capacitation treatment). A penetration assay was used in which spermatozoa had to travel 20 mm in a viscous medium (two media of differing viscosity: acrylamide and hyaluronic acid) through a tube device. The number of migrating spermatozoa, the physiology of the cells (motility analyzed using a CASA system; acrosomal status, viability and active mitochondria evaluated by flow cytometry; DNA fragmentation index calculated by quantitative PCR) and the morphometry of sperm heads (performed using an image analysis system) were evaluated after long-distance sperm migration. Ram sperm capacitation significantly stimulates cell migration through viscous media under geotactic conditions, and this effect is enhanced by progesterone induction. The rheological characteristics of viscous media have a marked impact on ram sperm migration, and acrylamide more favorably facilitates navigation over a large distance. The migrating spermatozoa are morphologically better adapted (high ellipticity) for displacement in viscous media and exhibit remarkably depleted mitochondrial membrane potential.
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/ MICINN/ Programa Nacional de Investigación Fundamenta/ AGL2008-03087/ES/ DISEÑO DE UN DISPOSITIVO PARA LA EVALUACION IN VITRO DE DAÑOS CELULARES DURANTE EL TRANSPORTE EN EL CERVIX DE SEMEN OVINO //
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