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Título
Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Scientific Reports
Número de la revista
1
Datos de la obra
Fernández-Veiga, L., Fuertes, M., Geijo, M. V., Pérez de Val, B., Vidal, E., Michelet, L., Boschiroli, M. L., Gómez-Buendía, A., Bezos, J., Jones, G. J., Vordermeier, M., Juste, R. A., Garrido, J. M., & Sevilla, I. A. (2023). Differences in skin test reactions to official and defined antigens in guinea pigs exposed to non-tuberculous and tuberculous bacteria. Scientific Reports, 13(1). https://doi.org/10.1038/S41598-023-30147-4
Editor
Nature research
Fecha
2023
Résumé
[EN] The single and comparative intradermal tuberculin tests (SITT and CITT) are official in vivo tests for bovine tuberculosis (TB) diagnosis using bovine and avian purified protein derivatives (PPD-B and PPD-A). Infection with bacteria other than Mycobacterium tuberculosis complex (MTC) can result in nonspecific reactions to these tests. We evaluated the performance of the skin test with PPDs and new defined antigens in the guinea pig model. A standard dose (SD) of Rhodococcus equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis, M. avium subsp. avium, M. avium subsp. hominissuis, M. scrofulaceum, M. persicum, M. microti, M. caprae and M. bovis, and a higher dose (HD) of M. nonchromogenicum, M. monacense, M. intracellulare, M. avium subsp. paratuberculosis were tested using PPD-B, PPD-A, P22, ESAT-6-CFP-10-Rv3615c peptide cocktail long (PCL) and fusion protein (FP). The SD of R. equi, Nocardia sp., M. nonchromogenicum, M. monacense, M. intracellulare and M. avium subsp. paratuberculosis did not cause any reactions. The HD of M. nonchromogenicum, M. monacense, M. intracellulare, and M. avium subsp. paratuberculosis and the SD of M. avium subsp. hominissuis, M. scrofulaceum and M. persicum, caused nonspecific reactions (SIT). A CITT interpretation would have considered M. avium complex and M. scrofulaceum groups negative, but not all individuals from M. nonchromogenicum HD, M. monacense HD and M. persicum SD groups. Only animals exposed to M. bovis and M. caprae reacted to PCL and FP. These results support the advantage of complementing or replacing PPD-B to improve specificity without losing sensitivity
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- info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i /PID2019-105155RB-C33/DEVELOPMENT OF INACTIVATED VACCINE PROTOTYPES FOR THE CONTROL OF TB IN DOMESTIC ANIMALS AND ASSESSMENT OF THE INTEREFERENCE CAUSED BY NON TUBERCULOUS ORGANISMS ON ITS DIAGNOSI//
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