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Título
Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium subspecies paratuberculosis in vitro
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Veterinary Research
Número de la revista
1
Cita Bibliográfica
Criado, M., Pérez, V., Arteche-Villasol, N., Elguezabal, N., Molina, E., Benavides, J., & Gutiérrez-Expósito, D. (2023). Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium subspecies paratuberculosis in vitro. Veterinary research, 54(1), 61. https://doi.org/10.1186/S13567-023-01193-7
Editorial
BMC
Fecha
2023
Resumen
[EN] Neutrophils constitute an essential component of the innate immune response, readily killing most bacteria through phagocytosis, degranulation, and the release of neutrophil extracellular traps (NETs) among other mechanisms. These cells play an unclear role in mycobacterial infections such as Mycobacterium avium subspecies paratuberculosis (Map), the etiological agent of paratuberculosis, and its response is particularly understudied in ruminants. Herein, a wide set of techniques were adapted, or newly developed, to study the in vitro response of caprine neutrophils after Map infection. Immunofluorescence was used to demonstrate, simultaneously, chemotaxis, phagocytosis, degranulation, and NETs. The quantification of neutrophil phagocytic activity against Map at a 1:10 multiplicity of infection (MOI), through flow cytometry, showed values that varied from 4.54 to 5.63% of phagocyting neutrophils. By immunofluorescence, a 73.3 ± 14.5% of the fields showed NETs, and the mean release of DNA, attributable to NETosis, calculated through a fluorometric method, was 16.2 ± 3.5%. In addition, the RNA expression of TGF-β, TNF and IL-1β cytokines, measured through reverse transcription qPCR, was significantly higher in the two latter. Overall, neutrophil response was proportional to the number of bacteria. This work confirms that the simultaneous study of several neutrophil mechanisms, and the combination of different methodologies, are essential to reach a comprehensive understanding of neutrophil response against pathogens, demonstrates that, in vitro, caprine neutrophils display a strong innate response against Map, using their entire repertoire of effector functions, and sets the basis for further in vitro and in vivo studies on the role of neutrophils in paratuberculosis.
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- info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-099496-B-I00/ES/MECANISMOS DE RESISTENCIA NATURAL E INDUCIDA POR LA VACUNACION FRENTE A LA PARATUBERCULOSIS//
- info:eu-repo/grantAgreement/AEI/Programa Estatal para Impulsar la Investigación Científico-Técnica y su Transferencia/PID2021-125807OB-C21/RTI2018-099496-B-I00/ES/ RESPUESTA INMUNITARIA E INMUNIDAD ENTRENADA EN LAS FASES INICIALES DE LA PARATUBERCULOSIS EN LA ESPECIE OVINA: VACUNACION E INFECCION//
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