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Título
Light-dependent inhibition of clathrin-mediated endocytosis in yeast unveils conserved functions of the AP2 complex
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
iScience
Número de la revista
10
Datos de la obra
Prischich, D., Camarero, N., Encinar del Dedo, J., Cambra-Pellejà, M., Prat, J., Nevola, L., Martín-Quirós, A., Rebollo, E., Pastor, L., Giralt, E., Geli, M. I., & Gorostiza, P. (2023). Light-dependent inhibition of clathrin-mediated endocytosis in yeast unveils conserved functions of the AP2 complex. iScience, 26(10). https://doi.org/10.1016/J.ISCI.2023.107899
Editor
Cell Press
Fecha
2023
ISSN
2589-0042
Résumé
[EN] Clathrin-mediated endocytosis (CME) is an essential cellular process, conserved among eukaryotes. Yeast constitutes a powerful genetic model to dissect the complex endocytic machinery, yet there is a lack of specific pharmacological agents to interfere with CME in these organisms. TL2 is a light-regulated peptide inhibitor targeting the AP2-β-adaptin/β-arrestin interaction and that can photocontrol CME with high spatiotemporal precision in mammalian cells. Here, we study endocytic protein dynamics by live-cell imaging of the fluorescently tagged coat-associated protein Sla1-GFP, demonstrating that TL2 retains its inhibitory activity in S. cerevisiae spheroplasts. This is despite the β-adaptin/β-arrestin interaction not being conserved in yeast. Our data indicate that the AP2 α-adaptin is the functional target of activated TL2. We identified as interacting partners for the α-appendage, the Eps15 and epsin homologues Ede1 and Ent1. This demonstrates that endocytic cargo loading and sensing can be executed by conserved molecular interfaces, regardless of the proteins involved
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Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i / PID2019-111493RB-I00/ES/ NEUROMODULACION DE LAS VIAS INHIBITORIAS MEDIANTE FOTOFARMACOLOGIA ACTIVADA POR LUZ ROJA E INFRARROJA//
- info:eu-repo/grantAgreement/AEI/ Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/BFU2017-82959-P/ES/ MECANISMOS MOLECULARES DEL TRAFICO ENDOCITICO//
- info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i / PID2020-120053GB-I00/ES/ REGULACION DE COMPLEJOS MACROMOLECULARES TRANSITORIOS POR ESTEROLES, FOSFOINOSITOLES Y CK2//
- info:eu-repo/grantAgreement/ MINECO / Programa Estatal de Promoción del Talento y su Empleabilidad/ BES-2015-072657/ES/ BES-2015-072657//
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