Compartir
Título
Inhibition of zygotic DNA repair: transcriptome analysis of the offspring in trout (Oncorhynchus mykiss)
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Reproduction
Número de la revista
1
Datos de la obra
Fernández-Díez, C., González-Rojo, S., Montfort, J., Le Cam, A., Bobe, J., Robles, V., Pérez-Cerezales, S., & Herráez, M. P. (2015). Inhibition of zygotic DNA repair: transcriptome analysis of the off spring in trout (Oncorhynchus mykiss). Reproduction, 149(1), 101–111. https://doi.org/10.1530/REP-14-0382
Editor
Society for Reproduction and Fertility
Fecha
2015
ISSN
1470-1626
Zusammenfassung
[EN] Zygotic repair of the paternal genome is a key event after fertilization. Spermatozoa accumulate DNA strand breaks during spermatogenesis and can suffer additional damage by different factors, including cryopreservation. Fertilization with DNA-damaged spermatozoa (DDS) is considered to promote implantation failures and abortions, but also long-term effects on the progeny that could be related with a defective repair. Base excision repair (BER) pathway is considered the most active in zygotic DNA repair, but healthy oocytes contain enzymes for all repairing pathways. In this study, the effects of the inhibition of the BER pathway in the zygote were analyzed on the progeny obtained after fertilization with differentially DDS. Massive gene expression (GE; 61 657 unique probes) was analyzed after hatching using microarrays. Trout oocytes are easily fertilized with DDS and the high prolificacy allows live progeny to be obtained even with a high rate of abortions. Nevertheless, the zygotic inhibition of Poly (ADP-ribose) polymerase, upstream of BER pathway, resulted in 810 differentially expressed genes (DEGs) after hatching. DEGs are related with DNA repair, apoptosis, telomere maintenance, or growth and development, revealing a scenario of impaired DNA damage signalization and repair. Downregulation of the apoptotic cascade was noticed, suggesting a selection of embryos tolerant to residual DNA damage during embryo development. Our results reveal changes in the progeny from defective repairing zygotes including higher malformations rate, weight gain, longer telomeres, and lower caspase 3/7 activity, whose long-term consequences should be analyzed in depth
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/Junta de Castilla y León/Programa Estatal de Fomento de la Investigación Científica y Técnica de Excelencia/LE365A11-2/Alteraciones en el material genético de espermatozoides: estudio de las consecuencias sobre la progenie en peces
- info:eu-repo/grantAgreement/MINECO/Plan Nacional de I+D+i/ AGL2011-27787/Análisis de la cromatina espermática de peces y sus aportaciones al control del desarrollo y la calidad de la descendencia
URI
DOI
Versión del editor
Aparece en las colecciones
- Untitled [5086]
Dateien zu dieser Ressource
Tamaño:
359.3
xmlui.dri2xhtml.METS-1.0.size-kilobytes
Formato:
Adobe PDF