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dc.contributor | Facultad de Veterinaria | es_ES |
dc.contributor.author | Arteche Villasol, Noive | |
dc.contributor.author | Gutiérrez Expósito, Daniel | |
dc.contributor.author | Vallejo, Raquel | |
dc.contributor.author | Espinosa Cerrato, José | |
dc.contributor.author | Elguezabal, Natalia | |
dc.contributor.author | Ladero Auñón, Iraia | |
dc.contributor.author | Royo, Marcos | |
dc.contributor.author | Ferreras Estrada, María Del Carmen | |
dc.contributor.author | Benavides, Julio | |
dc.contributor.author | Pérez Pérez, Valentín | |
dc.contributor.other | Sanidad Animal | es_ES |
dc.date | 2021 | |
dc.date.accessioned | 2024-03-13T09:35:45Z | |
dc.date.available | 2024-03-13T09:35:45Z | |
dc.identifier.citation | Arteche-Villasol, N., Gutiérrez-Expósito, D., Vallejo, R., Espinosa, J., Elguezabal, N., Ladero-Auñon, I., Royo, M., Ferreras Estrada, M. d. C., Benavides, J., & Pérez, V. (2021). Early response of monocyte-derived macrophages from vaccinated and non-vaccinated goats against in vitro infection with Mycobacterium avium subsp. paratuberculosis. Veterinary Research, 52(1). https://doi.org/10.1186/S13567-021-00940-Y | es_ES |
dc.identifier.other | https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-021-00940-y | es_ES |
dc.identifier.uri | https://hdl.handle.net/10612/18880 | |
dc.description.abstract | [EN]Paratuberculosis is a disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). Vaccination is the most cost-effective control method. However, despite the fact that macrophages are the main target cells for this pathogen, the precise mechanisms behind the response of the macrophage to Map infection and how it is modified by vaccination are yet poorly understood. The aim of this study was to investigate the effect of Silirum® vaccination in the early immune response of caprine monocyte-derived macrophages (CaMØs). Peripheral blood mononuclear cells (PBMCs) were obtained from vaccinated and non-vaccinated goats, cultured in vitro until differentiation to macrophages and infected with Map. After a 24 h incubation, Map viability and DNA were assessed in culture by viable colony count and real time quantitative polymerase chain reaction (qPCR). In addition, Map phagocytosis and expression of IL-10, IL-12, IFN-γ, TNF-α, IL-17A, IL-1β, iNOS, IL-6 and MIP-1β were also evaluated through immunofluorescence labelling and reverse transcriptase qPCR (RT-qPCR), respectively. A significant reduction of Map viability was observed in both supernatants (P < 0.05) and CaMØs (P < 0.001) from the vaccinated group. Similarly, the percentage of infected CaMØs and the number of internalized Map by CaMØs (P < 0.0001) was higher in the vaccinated group. Finally, iNOS (P < 0.01) and IL-10 were significantly up-regulated in CaMØs from vaccinated goats, whereas only MIP-1β was up-regulated in non-vaccinated animals (P < 0.05). These results show that vaccination modifies the immune response of CaMØs, suggesting that the phagocytosis and microbiocidal activity of macrophages against Map is enhanced after vaccination. | es_ES |
dc.language | spa | es_ES |
dc.publisher | BMC | es_ES |
dc.rights | Atribución 4.0 Internacional | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | Sanidad animal | es_ES |
dc.subject | Veterinaria | es_ES |
dc.subject.other | Map | es_ES |
dc.subject.other | Vaccination | es_ES |
dc.subject.other | Caprine monocyte-derived macrophages | es_ES |
dc.subject.other | Viability | es_ES |
dc.subject.other | Phagocytosis | es_ES |
dc.title | Early response of monocyte-derived macrophages from vaccinated and non-vaccinated goats against in vitro infection with Mycobacterium avium subsp. paratuberculosis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1186/s13567-021-00940-y | |
dc.description.peerreviewed | SI | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/AGL2015-66540-C2-1-R/ES/RESPUESTA INMUNE Y MARCADORES DE PROTECCION ASOCIADOS A LA VACUNACION EN PARATUBERCULOSIS | es_ES |
dc.relation.projectID | info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-099496-B-I00/ES/MECANISMOS DE RESISTENCIA NATURAL E INDUCIDA POR LA VACUNACION FRENTE A LA PARATUBERCULOSIS | es_ES |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | es_ES |
dc.identifier.essn | 1297-9716 | |
dc.journal.title | Veterinary Research | es_ES |
dc.volume.number | 52 | es_ES |
dc.issue.number | 1 | es_ES |
dc.page.initial | 69 | es_ES |
dc.type.hasVersion | info:eu-repo/semantics/publishedVersion | es_ES |
dc.subject.unesco | 3109 Ciencias Veterinarias | es_ES |
dc.subject.unesco | 3109.05 Microbiología | es_ES |
dc.subject.unesco | 3109.05 Microbiología | es_ES |
dc.subject.unesco | 2412.10 Vacunas | es_ES |
dc.description.project | The Spanish Ministry of Science and Innovation (Projects AGL2015-66540-C2-1-R and RTI2018-099496-B-I00) and Junta de Castilla y León (Project LE259P18) financed this work. This work was also financially supported by the University of León. Noive Arteche Villasol has been financially supported by a predoctoral contract (BES-2016-076513) from the Spanish Ministry of Science and Innovation. Daniel Gutiérrez-Expósito and José Espinosa are the recipients of a postdoctoral contract from the Ministry of Science and Innovation (Grants No. FJCI-2017-32020 and FJC2019-042422-I, respectively). Iraia Ladero-Auñon holds a pre-doctoral grant from “Departamento de Economía e Infraestructuras” of the Basque Government. | es_ES |
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