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Título
Toxicity of Recombinant Necrosis and Ethylene-Inducing Proteins (NLPs) from Neofusicoccum parvum
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Toxins
Número de la revista
4
Datos de la obra
Pour, F. N., Cobos, R., Coque, J. J. R., Serôdio, J., Alves, A., Félix, C., Ferreira, V., Esteves, A. C., & Duarte, A. S. (2020). Toxicity of recombinant necrosis and ethylene-inducing proteins (NLPs) from Neofusicoccum parvum. Toxins, 12(4), Article e235. https://doi.org/10.3390/TOXINS12040235
Editor
MDPI
Fecha
2020
Zusammenfassung
[EN] Neofusicoccum parvum is a fungal pathogen associated with a wide range of plant hosts. Despite being widely studied, the molecular mechanism of infection of N. parvum is still far from being understood. Analysis of N. parvum genome lead to the identification of six putative genes encoding necrosis and ethylene-inducing proteins (NLPs). The sequence of NLPs genes (NprvNep 1-6) were analyzed and four of the six NLP genes were successfully cloned, expressed in E. coli and purified by a nity chromatography. Pure recombinant proteins were characterized according to their phytotoxic and cytotoxic e ects to tomato leaves and to mammalian Vero cells, respectively. These assays revealed that all NprvNeps tested are cytotoxic to Vero cells and also induce cell death in tomato leaves. NprvNep2 was the most toxic to Vero cells, followed by NprvNep1 and 3. NprvNep4 induced weaker, but, nevertheless, still significant toxic e ects to Vero cells. A similar trend of toxicity was observed in tomato leaves: the most toxic was NprvNep 2 and the least toxic NprvNep 4. This study describes for the first time an overview of the NLP gene family of N. parvum and provides additional insights into its pathogenicity mechanism
Materia
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Peer review
SI
URI
DOI
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