Compartir
Título
Possible Mechanisms of Action of Two Pseudomonas fluorescens Isolates as Probiotics on Saprolegniosis Control in Rainbow Trout (Oncorhynchus mykiss Walbaum)
Autor
Facultad/Centro
Área de conocimiento
Título de la revista
Animals
Número de la revista
9
Datos de la obra
González-Palacios, C., Fregeneda-Grandes, J.-M., & Aller-Gancedo, J.-M. (2020). Possible mechanisms of action of two pseudomonas fluorescens isolates as probiotics on saprolegniosis control in rainbow trout (Oncorhynchus mykiss walbaum). Animals, 10(9), Article e1507. https://doi.org/10.3390/ANI10091507
Editor
MDPI
Fecha
2020
Abstract
[EN] Probiotics have been proposed as one of the alternatives to the chemical treatments currently used in aquaculture. Recently, the possible usefulness of certain microorganisms, mainly bacteria, has been highlighted as a potential biocontrol for saprolegniosis. In the present work we investigated the possible mechanisms of action of two isolates of Pseudomonas fluorescens (LE89 and LE141) with proven ability to reduce Saprolegnia parasitica infection in rainbow trout under experimental conditions when they are added to the tank water. The stimulation of the innate immune response and the production of siderophores and bioactive substances inhibiting S. parasitica present in cells and supernatants of LE89 and LE141 were studied. Regarding the immune response the only noteworthy points were the increase in the phagocytic activity of macrophages and the concentration of serum proteins when LE141 was administered. Both bacteria produced siderophores. When analyzing the protein substances present in supernatants, it was observed that in both isolates the proteins with inhibitory activity present might be siderophores. In LE141, besides siderophores, a protein of 66 kDa was identified in the fraction responsible for inhibition. To sum up, the two P. fluorescens isolates might be usable for biocontrol of saprolegniosis and that the mode of action of these bacteria is likely to be related to the production of siderophores.
Materia
Palabras clave
Peer review
SI
ID proyecto
- info:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/AGL2010-18562/ES/LUCHA CONTRA LA SAPROLEGNIOSIS POR MEDIO DE BACTERIAS PROBIOTICAS//
URI
DOI
Versión del editor
Collections
- Untitled [5086]
Files in this item
Tamaño:
1.703
xmlui.dri2xhtml.METS-1.0.size-megabytes
Formato:
Adobe PDF